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Photosynthetic Gas Exchange in the Closed Ecosystem for Space. Phase II, Part II. Studies on the Growth of Thermophilic Chlorella 71105Bench-scale studies with Chlorella pyrenoidosa 71105 were conducted in four- and eight-liter culture vessels, with and without recycling. It was established that the algal strain could be maintained for periods up to 72 days with supplemented re-cycled medium. In a factorial series of experiments, the highest yields were obtained with a 2.0% concentration of carbon dioxide, a 0.07 dilution factor (nutrient dilution rate/culture volume), and an 8.5 ft(exp -1) ratio of light surface to liquid culture volume. Optimum pH and concentrations of the constituents of the growth medium were determined in corollary test-tube studies. Other test-tube studies showed that the nutrient medium used for mass culture could be stored up to five weeks at 25 C or at refrigerator temperature (4 C) with little change in nitrogen-urea level. Algal suspensions stored at 4 C remained viable for periods of at least 12 weeks; growth resumed after a lag period when stored suspensions were cultured at 39 C. Streptomycin was found suitable for controlling a blue-green containment.
Document ID
19620001732
Acquisition Source
Headquarters
Document Type
Other
Authors
Leone, Donald E. (General Dynamics Corp. Groton, CT, United States)
Date Acquired
August 1, 2013
Publication Date
August 1, 1961
Subject Category
Space Transportation And Safety
Report/Patent Number
HQ-E-DAA-TN
U411-61-106
Accession Number
62N11732
Funding Number(s)
CONTRACT_GRANT: NASW-95
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Keywords
ALGAE
CLOSED ECOLOGICAL SYSTEM
CHLORELLA
PHOTOSYNTHESIS
GAS EXCHANGE

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