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Analysis of results of ASTP experiment in electrophoresisThe Apollo-Soyuz Test Project (ASTP) included an electrophoretic separation experiment of biological cells. The nature separation results of aldehyde-fixed rabbit, human and horse red blood cells, which were taken in the form of photographs taken at three-minute intervals, are the subject of this report. The electrophoretic separation was successful in that fractionation according to mobility did occur and was found in the sliced samples. Photographic evidence indicates that the low electroosmotic methylcellulose coating was successful in reducing the electroosmosis to a near zero value. Also, the flight film shows that the bands migrated down the column as theory would predict, producing two bands of high cell concentration separated and surrounded by regions of lower cell concentration. However, most likely some clumping of cells occurred to cause the trailing band to be larger than expected from theory. Overall, the experiment was a success in demonstrating a static electrophoresis separation under microgravity conditions with a resolution not possible on earth.
Document ID
19780004225
Acquisition Source
Legacy CDMS
Document Type
Contractor Report (CR)
Authors
Vanderhoff, J. W. (Lehigh Univ. Bethlehem, PA, United States)
Micale, F. J. (Lehigh Univ. Bethlehem, PA, United States)
Krumrine, P. H. (Lehigh Univ. Bethlehem, PA, United States)
Date Acquired
September 3, 2013
Publication Date
July 1, 1977
Subject Category
Inorganic And Physical Chemistry
Report/Patent Number
NASA-CR-150469
Accession Number
78N12168
Funding Number(s)
CONTRACT_GRANT: NAS8-32124
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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