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Preparation of guinea pig macrophage for electrophoretic experiments in spaceMethods of storage and cultivation of macrophage cells in preparation for space experiments were investigated. Results show that freezing and thawing immediately after extraction did not cause any change in viability or electrophoretic mobility of the cells. A prolonged storage at -80 C did cause cell damage as indicated by a 95% reduction in variable cells. Cell damage was decreased when Glycerol or Dimethyl Sulfoxide (DMSO) was added as a cryogenic protective agent. A 100% viability was observed in cultivation experiments after two weeks due to the additional serum. Results from gamma-glutamyl transpeptidase study showed a zero activity rate. It is suggested that a flat stationary field be used for the collection and use of macrophage. It was found that a 24-hour delay in obtaining macrophage cells helps to maintain a pure culture.
Document ID
19790019643
Acquisition Source
Legacy CDMS
Document Type
Contractor Report (CR)
Date Acquired
September 3, 2013
Publication Date
May 1, 1979
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-158777
Accession Number
79N27814
Funding Number(s)
CONTRACT_GRANT: NSG-8062
CONTRACT_GRANT: NSG-8064
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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