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Moving wall, continuous flow electronphoresis apparatusThis invention relates generally to electrophoresis devices and more particularly to a moving wall, continuous flow device in which an electrophoresis chamber is angularly positionable with respect to the direction of moving belt walls. A frame with an electrophoresis chamber is rotatably supported between two synchronously driven belt walls. This allows the chamber to be angularly positionable with respect to the direction of belt travel, which compensates for electroosmotic flow within the electrophoresis chamber. Injection of a buffer solution via an opening and a homogenous sample stream via another opening is performed at the end of a chamber, and collection of buffer and the fractionated species particles is done by a conventional collection array at an opposite end of the chamber. Belts are driven at a rate which exactly matches the flow of buffer and sample through the chamber, which entrains the buffer to behave as a rigid electrophoretic medium, eliminating flow distortions (Poiseuille effect). Additionally, belt material for each belt is stored at one end of the device and is taken up by drive wheels at an opposite end. The novelty of this invention particularly lies in the electrophoresis chamber being angularly positionable between two moving belt walls in order to compensate for electroosmotic flow. Additionally, new belt material is continuously exposed within the chamber, minimizing flow distortion due to contamination of the belt material by the sample.
Document ID
19880014461
Acquisition Source
Legacy CDMS
Document Type
Other - Patent
Authors
Rhodes, Percy H. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Snyder, Robert S. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 13, 2013
Publication Date
June 21, 1988
Subject Category
Inorganic And Physical Chemistry
Accession Number
88N23845
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Patent
NASA-CASE-MFS-28142-1|US-PATENT-4,752,372
Patent Application
US-PATENT-APPL-SN-904128

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