NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Collections About News Help Login
Back to Results
Insulin and insulin-like growth factor-1 induce pronounced hypertrophy of skeletal myofibers in tissue cultureSkeletal myofibers differentiated from primary avian myoblasts in tissue culture can be maintained in positive nitrogen balance in a serum-free medium for at least 6 to 7 days when embedded in a three dimensional collagen gel matrix. The myofibers are metabolically sensitive to physiological concentrations of insulin but these concentrations do not stimulate cell growth. Higher insulin concentrations stimulate both cell hyperplasia and myofiber hypertrophy. Cell growth results from a long term 42 percent increase in total protein synthesis and a 38 percent increase in protein degradation. Myofiber diameters increase by 71 to 98 percent after 6 to 7 days in insulin-containing medium. Insulin-like growth factor-1 but not insulin-like growth factor-2, at 250 ng/ml, is as effective as insulin in stimulating cell hyperplasia and myofiber hypertrophy. This model system provides a new method for studying the long-term anabolic effects of insulin and insulin-like growth factors on myofiber hypertrophy under defined tissue culture conditions.
Document ID
19900019644
Acquisition Source
Legacy CDMS
Document Type
Contractor Report (CR)
Authors
Vandenburgh, Herman H. (Miriam Hospital Providence, RI, United States)
Karlisch, Patricia (Miriam Hospital Providence, RI, United States)
Shansky, Janet (Miriam Hospital Providence, RI, United States)
Date Acquired
September 6, 2013
Publication Date
January 1, 1990
Subject Category
Life Sciences (General)
Report/Patent Number
NAS 1.26:187026
NASA-CR-187026
Accession Number
90N28960
Funding Number(s)
CONTRACT_GRANT: NAG2-414
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

Related Records

There are no records associated with this record.
No Preview Available