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Horizontally rotated cell culture system with a coaxial tubular oxygenatorThe present invention relates to a horizontally rotating bioreactor useful for carrying out cell and tissue culture. For processing of mammalian cells, the system is sterilized and fresh fluid medium, microcarrier beads, and cells are admitted to completely fill the cell culture vessel. An oxygen containing gas is admitted to the interior of the permeable membrane which prevents air bubbles from being introduced into the medium. The cylinder is rotated at a low speed within an incubator so that the circular motion of the fluid medium uniformly suspends the microbeads throughout the cylinder during the cell growth period. The unique design of this cell and tissue culture device was initially driven by two requirements imposed by its intended use for feasibility studies for three dimensional culture of living cells and tissues in space by JSC. They were compatible with microgravity and simulation of microgravity in one G. The vessels are designed to approximate the extremely quiescent low shear environment obtainable in space.
Document ID
19910021353
Acquisition Source
Legacy CDMS
Document Type
Other - Patent
Authors
Wolf, David A.
(Krug International Houston, TX., United States)
Schwarz, Ray P.
(Krug International Houston, TX., United States)
Trinh, Tinh T.
(Krug International Houston, TX., United States)
Date Acquired
August 14, 2013
Publication Date
June 25, 1991
Subject Category
Life Sciences (General)
Accession Number
91N30667
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Patent
US-PATENT-5,026,650|NASA-CASE-MSC-21294-1
Patent Application
US-PATENT-APPL-SN-213558
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