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Comparison of epifluorescent viable bacterial count methodsTwo methods, the 2-(4-Iodophenyl) 3-(4-nitrophenyl) 5-phenyltetrazolium chloride (INT) method and the direct viable count (DVC), were tested and compared for their efficiency for the determination of the viability of bacterial populations. Use of the INT method results in the formation of a dark spot within each respiring cell. The DVC method results in elongation or swelling of growing cells that are rendered incapable of cell division. Although both methods are subjective and can result in false positive results, the DVC method is best suited to analysis of waters in which the number of different types of organisms present in the same sample is assumed to be small, such as processed waters. The advantages and disadvantages of each method are discussed.
Document ID
19920021062
Acquisition Source
Legacy CDMS
Document Type
Technical Memorandum (TM)
Authors
Rodgers, E. B.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Huff, T. L.
(Sverdrup Technology, Inc., Huntsville AL., United States)
Date Acquired
September 6, 2013
Publication Date
July 1, 1992
Subject Category
Life Sciences (General)
Report/Patent Number
NAS 1.15:103592
NASA-TM-103592
Accession Number
92N30305
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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