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Convective flow effects on protein crystal growthDuring the fourth semi-annual period under this grant we have pursued the following activities: (1) crystal growth morphology and kinetics studies with tetragonal lysozyme. These clearly revealed the influence of higher molecular weight protein impurities on interface shape; (2) characterization of the purity and further purification of lysozyme solutions. These efforts have, for the first time, resulted in lysozyme free of higher molecular weight components; (3) continuation of the salt repartitioning studies with Seikagaku lysozyme, which has a lower protein impurity content that Sigma stock. These efforts confirmed our earlier findings of higher salt contents in smaller crystals. However, less salt is in corporated into the crystals grown from Seikagaku stock. This strongly suggests a dependence of salt repartitioning on the concentration of protein impurities in lysozyme. To test this hypothesis, repartitioning studies with the high purity lysozyme prepared in-house will be begun shortly; (4) numerical modelling of the interaction between bulk transport and interface kinetics. These simulations have produced interface shapes which are in good agreement with out experimental observations; and (5) light scattering studies on under- and supersaturated lysozyme solutions. A consistent interpretation of the static and dynamic data leaves little doubt that pre-nucleation clusters, claimed to exist even in undersaturated solutions, are not present. The article: 'Growth morphology response to nutrient and impurity nonuniformities' is attached.
Document ID
19950018114
Acquisition Source
Legacy CDMS
Document Type
Contractor Report (CR)
Authors
Rosenberger, Franz (Alabama Univ. Huntsville, AL, United States)
Monaco, Lisa A. (Alabama Univ. Huntsville, AL, United States)
Date Acquired
September 6, 2013
Publication Date
January 31, 1995
Subject Category
Solid-State Physics
Report/Patent Number
NASA-CR-197891
NAS 1.26:197891
Accession Number
95N24534
Funding Number(s)
CONTRACT_GRANT: NAG8-950
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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