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Mutagenesis in human cells with accelerated H and Fe ionsThe overall goals of this research were to determine the risks of mutation induction and the spectra of mutations induced by energetic protons and iron ions at two loci in human lymphoid cells. During the three year grant period the research has focused in three major areas: (1) the acquisition of sufficient statistics for human TK6 cell mutation experiments using Fe ions (400 MeV/amu), Fe ions (600 MeV/amu) and protons (250 MeV/amu); (2) collection of thymidine kinase- deficient (tk) mutants or hypoxanthine phosphoribosyltransferase deficient (hprt) mutants induced by either Fe 400 MeV/amu, Fe 600 MeV/amu, or H 250 MeV/amu for subsequent molecular analysis; and (3) molecular characterization of mutants isolated after exposure to Fe ions (600 MeV/amu). As a result of the shutdown of the BEVALAC heavy ion accelerator in December 1992, efforts were rearranged somewhat in time to complete our dose-response studies and to complete mutant collections in particular for the Fe ion beams prior to the shutdown. These goals have been achieved. A major effort was placed on collection, re-screening, and archiving of 3 different series of mutants for the various particle beam exposures: tk-ng mutants, tk-sg mutants, and hprt-deficient mutants. Where possible, groups of mutants were isolated for several particle fluences. Comparative analysis of mutation spectra has occured with characterization of the mutation spectrum for hprt-deficient mutants obtained after exposure of TK6 cells to Fe ions (600 MeV/amu) and a series of spontaneous mutants.
Document ID
19950021756
Acquisition Source
Legacy CDMS
Document Type
Contractor Report (CR)
Authors
Kronenberg, Amy (California Univ. Berkeley. Lawrence Berkeley Lab, CA, United States)
Date Acquired
September 6, 2013
Publication Date
April 1, 1994
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-198599
NAS 1.26:198599
Accession Number
95N28177
Funding Number(s)
CONTRACT_GRANT: NASA ORDER T-9309-R
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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