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A Fluorescence Recovery After Photobleaching (FRAP) Technique for the Measurement of Solute Transport Across Surfactant-Laden InterfacesThe technique of Fluorescence Recovery After Photobleaching (FRAP) has been applied to the measurement of interfacial transport in two-phase systems. FRAP exploits the loss of fluorescence exhibited by certain fluorophores when over-stimulated (photobleached), so that a two-phase system, originally at equilibrium, can be perturbed without disturbing the interface by strong light from an argon-ion laser and its recovery monitored by a microscope-mounted CCD camera as it relaxes to a new equilibrium. During this relaxation, the concentration profiles of the probe solute are measured on both sides of the interface as a function of time, yielding information about the transport characteristics of the system. To minimize the size of the meniscus between the two phases, a photolithography technique is used to selectively treat the glass walls of the cell in which the phases are contained. This allows concentration measurements to be made very close to the interface and increases the sensitivity of the FRAP technique.
Document ID
19970000462
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Browne, Edward P.
(Massachusetts Inst. of Tech. Cambridge, MA United States)
Hatton, T. Alan
(Massachusetts Inst. of Tech. Cambridge, MA United States)
Date Acquired
August 17, 2013
Publication Date
September 1, 1996
Publication Information
Publication: Third Microgravity Fluid Physics Conference
Subject Category
Fluid Mechanics And Heat Transfer
Accession Number
97N10429
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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