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Determination of (alpha)-dialkylamino acids and their Enantiomers in Geological Samples by High-Performance Liquid Chromatography after Dervatization with a Chiral Adduct of (omicron)-PhthaldialdehydeDerivatization with (omicron)-phthaldialdehyde (OPA) and the chiral thiol N-acetyl-L-cysteine (NAC) is a convenient and sensitive technique for the HPLC detection and resolution of protein amino acid enantiomers. The kinetics of the reaction of OPA-NAC with (alpha)-dialkylamino acids was investigated. The fluorescence yield of (alpha)-dialkylamino acids was only about 10% of that of protein amino acids when the derivatization was carried out at room temperature for 1-2 min, which is the procedure generally used for protein amino acid analyses. The fluorescence yield of (alpha)-dialkylamino acids can be enhanced by up to ten-fold when the derivatization reaction time is increased to 15 min at room temperature. The OPA-NAC technique was optimized for the detection and enantiomeric resolution of a-dialkylamino acids in geological samples which contain a large excess of protein amino acids. The estimated detection limit for a-dialkylamino acids is 1-2 pmol, comparable to that for protein amino acids.
Document ID
19980032585
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Zhoa, Meixun
(California Univ., San Diego La Jolla, CA United States)
Bada, Jeffrey L.
(California Univ., San Diego La Jolla, CA United States)
Date Acquired
September 6, 2013
Publication Date
January 1, 1995
Publication Information
Publication: Journal of Chromatography A
Publisher: Elsevier Science Publishers Ltd.
Volume: 690
ISSN: 0021-9673
Subject Category
Chemistry And Materials (General)
Report/Patent Number
NASA/CR-95-207590
NAS 1.26:207590
Funding Number(s)
CONTRACT_GRANT: NSF EAR-89-15829
CONTRACT_GRANT: NAGw-2881
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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