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The Limits of Template-Directed Synthesis with Nucleoside-5'-Phosphoro(2-Methyl) ImidazolidesIn earlier work we have shown that C-rich templates containing isolated A, T or G residues and short oligo(G) sequences can be copied effectively using nucleoside-5'-phosphoro(2-methyl)imidazolides as substrates. We now show that isolated A or T residues within an oligo(G) sequence are a complete block to copying and that an isolated C residue is copied inefficiently. Replication is possible only if there are two complementary oligonucleotides each of which acts as a template to facilitate the synthesis of the other. We emphasize the severity of the problems that need to be overcome to make possible non-enzymatic replication in homogeneous aqueous solution. We conclude that an efficient catalyst was involved in the origin of polynucleotide replication.
Document ID
19980033152
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
External Source(s)
doi:10.1007/BF01582078
Authors
Hill, Aubrey R., Jr. (Salk Inst. for Biological Studies San Diego, CA United States)
Orgel, Leslie E. (Salk Inst. for Biological Studies San Diego, CA United States)
Wu, Taifeng (Northwestern Univ. Evanston, IL United States)
Date Acquired
September 6, 2013
Publication Date
January 1, 1993
Publication Information
Publication: Origins of Life and Evolution of the Biosphere
Publisher: Kluwer Academic Publishers
Volume: 23
Subject Category
Chemistry And Materials (General)
Report/Patent Number
NAS 1.26:204936
NASA-CR-204936
Funding Number(s)
CONTRACT_GRANT: NAGw-1660
Distribution Limits
Public
Copyright
Public Use Permitted.

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