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Satellite cell proliferation in adult skeletal muscleNovel methods of retroviral-mediated gene transfer for the in vivo corporation and stable expression of eukaryotic or prokaryotic foreign genes in tissues of living animals is described. More specifically, methods of incorporating foreign genes into mitotically active cells are disclosed. The constitutive and stable expression of E. coli .beta.-galactosidase gene under the promoter control of the Moloney murine leukemia virus long terminal repeat is employed as a particularly preferred embodiment, by way of example, establishes the model upon which the incorporation of a foreign gene into a mitotically-active living eukaryotic tissue is based. Use of the described methods in therapeutic treatments for genetic diseases, such as those muscular degenerative diseases, is also presented. In muscle tissue, the described processes result in genetically-altered satellite cells which proliferate daughter myoblasts which preferentially fuse to form a single undamaged muscle fiber replacing damaged muscle tissue in a treated animal. The retroviral vector, by way of example, includes a dystrophin gene construct for use in treating muscular dystrophy. The present invention also comprises an experimental model utilizable in the study of the physiological regulation of skeletal muscle gene expression in intact animals.
Document ID
20080004741
Acquisition Source
Ames Research Center
Document Type
Other - Patent
Authors
Booth, Frank W.
Thomason, Donald B.
Morrison, Paul R.
Stancel, George M.
Date Acquired
August 24, 2013
Publication Date
November 14, 1995
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NAG2-239
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
Patent
US-PATENT-5,466,676
Patent Application
US-PATENT-APPL-SN-823783

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