NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Isolation of Precursor Cells from Waste Solid Fat TissueA process for isolating tissue-specific progenitor cells exploits solid fat tissue obtained as waste from such elective surgical procedures as abdominoplasties (tummy tucks) and breast reductions. Until now, a painful and risky process of aspiration of bone marrow has been used to obtain a limited number of tissue- specific progenitor cells. The present process yields more tissue-specific progenitor cells and involves much less pain and risk for the patient. This process includes separation of fat from skin, mincing of the fat into small pieces, and forcing a fat saline mixture through a sieve. The mixture is then digested with collagenase type I in an incubator. After centrifugation tissue-specific progenitor cells are recovered and placed in a tissue-culture medium in flasks or Petri dishes. The tissue-specific progenitor cells can be used for such purposes as (1) generating three-dimensional tissue equivalent models for studying bone loss and muscle atrophy (among other deficiencies) and, ultimately, (2) generating replacements for tissues lost by the fat donor because of injury or disease.
Document ID
20090020558
Acquisition Source
Johnson Space Center
Document Type
Other - NASA Tech Brief
Authors
Byerly, Diane
(NASA Johnson Space Center Houston, TX, United States)
Sognier, Marguerite A.
(Universities Space Research Association Houston, TX, United States)
Date Acquired
August 24, 2013
Publication Date
May 1, 2009
Publication Information
Publication: NASA Tech Briefs, May 2009
Subject Category
Life Sciences (General)
Report/Patent Number
MSC-23775-1
Distribution Limits
Public
Copyright
Public Use Permitted.
No Preview Available