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Multiplexed Colorimetric Solid-Phase ExtractionMultiplexed colorimetric solid-phase extraction (MC-SPE) is an extension of colorimetric solid-phase extraction (C-SPE) an analytical platform that combines colorimetric reagents, solid phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water. In CSPE, analytes are extracted and complexed on the surface of an extraction membrane impregnated with a colorimetric reagent. The analytes are then quantified directly on the membrane surface using a handheld diffuse reflectance spectrophotometer. Importantly, the use of solid-phase extraction membranes as the matrix for impregnation of the colorimetric reagents creates a concentration factor that enables the detection of low concentrations of analytes in small sample volumes. In extending C-SPE to a multiplexed format, a filter holder that incorporates discrete analysis channels and a jig that facilitates the concurrent operation of multiple sample syringes have been designed, enabling the simultaneous determination of multiple analytes. Separate, single analyte membranes, placed in a readout cartridge create unique, analyte-specific addresses at the exit of each channel. Following sample exposure, the diffuse reflectance spectrum of each address is collected serially and the Kubelka-Munk function is used to quantify each water quality parameter via calibration curves. In a demonstration, MC-SPE was used to measure the pH of a sample and quantitate Ag(I) and Ni(II).
Document ID
20090040077
Acquisition Source
Johnson Space Center
Document Type
Other - NASA Tech Brief
Authors
Gazda, Daniel B.
(Iowa State Univ. IA, United States)
Fritz, James S.
(Iowa State Univ. IA, United States)
Porter, Marc D.
(Iowa State Univ. IA, United States)
Date Acquired
August 24, 2013
Publication Date
November 1, 2009
Publication Information
Publication: NASA Tech Briefs, November 2009
Subject Category
Technology Utilization And Surface Transportation
Report/Patent Number
MSC-23850-1
Distribution Limits
Public
Copyright
Public Use Permitted.
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