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Detergent-Specific Membrane Protein Crystallization ScreensA suite of reagents has been developed for three-dimensional crystallization of integral membranes present in solution as protein-detergent complexes (PDCs). The compositions of these reagents have been determined in part by proximity to the phase boundaries (lower consolute boundaries) of the detergents present in the PDCs. The acquisition of some of the requisite phase-boundary data and the preliminary design of several of the detergent- specific screens was supported by a NASA contract. At the time of expiration of the contract, a partial set of preliminary screens had been developed. This work has since been extended under non-NASA sponsorship, leading to near completion of a set of 20 to 30 different and unique detergent- specific 96-condition screens.
Document ID
20100002868
Acquisition Source
Marshall Space Flight Center
Document Type
Other - NASA Tech Brief
External Source(s)
http://www.techbriefs.com/component/content/article/2090
Authors
Wiener, Michael (Virginia Univ. Charlottesville, VA, United States)
Date Acquired
August 25, 2013
Publication Date
August 1, 2007
Publication Information
Publication: NASA Tech Briefs, August 2007
Subject Category
Man/System Technology And Life Support
Report/Patent Number
MFS-32436-1
Distribution Limits
Public
Copyright
Public Use Permitted.

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IDRelationTitle20100002858Collected WorksNASA Tech Briefs, August 2007
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