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Effects of Bone Morphogenic Proteins on Engineered CartilageA report describes experiments on the effects of bone morphogenic proteins (BMPs) on engineered cartilage grown in vitro. In the experiments, bovine calf articular chondrocytes were seeded onto biodegradable polyglycolic acid scaffolds and cultured in, variously, a control medium or a medium supplemented with BMP-2, BMP-12, or BMP-13 in various concentrations. Under all conditions investigated, cell-polymer constructs cultivated for 4 weeks macroscopically and histologically resembled native cartilage. At a concentration of 100 ng/mL, BMP-2, BMP-12, or BMP-13 caused (1) total masses of the constructs to exceed those of the controls by 121, 80, or 62 percent, respectively; (2) weight percentages of glycosaminoglycans in the constructs to increase by 27, 18, or 15, respectively; and (3) total collagen contents of the constructs to decrease to 63, 89, or 83 percent of the control values, respectively. BMP-2, but not BMP-12 or BMP-13, promoted chondrocyte hypertrophy. These observations were interpreted as suggesting that the three BMPs increase the growth rates and modulate the compositions of engineered cartilage. It was also concluded that in vitro engineered cartilage is a suitable system for studying effects of BMPs on chondrogenesis in a well-defined environment.
Document ID
20100010933
Acquisition Source
Johnson Space Center
Document Type
Other - NASA Tech Brief
External Source(s)
http://www.techbriefs.com/component/content/article/2202
Authors
Gooch, Keith, J. (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Blunk, Torsten (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Courter, Donald L. (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Sieminski, Alisha (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Vunjak-Novakovic, Gordana (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Freed, Lisa E. (Massachusetts Inst. of Tech. Cambridge, MA, United States)
Date Acquired
August 24, 2013
Publication Date
September 1, 2007
Publication Information
Publication: NASA Tech Briefs, September 2007
Subject Category
Man/System Technology And Life Support
Report/Patent Number
MSC-23647
Distribution Limits
Public
Copyright
Public Use Permitted.

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