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Karyotyping of Chromosomes in Human Bronchial Epithelial Cells Transformed by High Energy Fe IonsLung cancer induced from exposure to space radiation is believed to be one of the most significant health risks for long-term space travels. In a previous study, normal human bronchial epithelial cells (HBECs), immortalized through the expression of Cdk4 and hTERT, were exposed to gamma rays and high energy Fe ions for the selection of transformed clones induced by low- and high-LET radiation. In this research, we analyzed chromosome aberrations in these selected clones for genomic instability using the multi-color fluorescent in situ hybridization (mFISH), as well as the multi-banding in situ hybridization (mBAND) techniques. In most of the clones, we found chromosomal aberrations involving translocations between different chromosomes, with several of the breaks occurred in the q-arm of chromosome 3. We also identified copy number variations between the transformed clones and the parental HBEC cells regardless of the exposure condition. Our results indicated that the chromosomal aberrations in low- and high radiation-induced transformed clones are inadequately different from spontaneous soft agar growth. Further analysis is underway to reveal the genomic instability in more transformed clones
Document ID
20140013491
Acquisition Source
Johnson Space Center
Document Type
Conference Paper
Authors
Yeshitla, Samrawit
(NASA Johnson Space Center Houston, TX, United States)
Zhang, Ye
(Wyle Labs., Inc. Houston, TX, United States)
Park, Seongmi
(University of Southwestern Texas Medical School Dallas, TX, United States)
Story, Michael T.
(University of Southwestern Texas Medical School Dallas, TX, United States)
Wilson, Bobby
(Texas Southern Univ. Houston, TX, United States)
Wu, Honglu
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
November 14, 2014
Publication Date
January 1, 2014
Subject Category
Aerospace Medicine
Report/Patent Number
JSC-CN-32211
Meeting Information
Meeting: NASA''s Human Research Program Investgator''s Workshop
Location: Galveston, TX
Country: United States
Start Date: January 13, 2015
End Date: January 15, 2015
Sponsors: National Space Biomedical Research Inst., NASA Headquarters
Distribution Limits
Public
Copyright
Public Use Permitted.
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