User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

A validated method for the quantification of pimarane and trachylobane diterpenes in the leaves of Croton zambesicus by capillary gas chromatography

Primary tabs

download
  • Open access
  • PDF
  • 289.59 K
Block, Sébastien [UCL] Brkic, Dejan [UCL] Hubert, Philippe Quetin-Leclercq, Joëlle [UCL]

A sensitive and accurate method, combining Soxhlet extraction, solid-phase extraction and capillary gas chromatography, is described for the quantitative determination of four new diterpenes (ent-trachyloban-3beta-ol, ent-18-hydroxy-trachyloban-3-one, ent-trachyloban-3-one and isopimara-7,15-dien-3beta-ol) from the leaves of Croton zambesicus. This is the first method describing the quantification of trachylobane diterpenes in a crude extract. It has been fully validated in order to be able to compare the diterpene composition in other samples of C. zambesicus, which is an important source of trachylobanes.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2005
Language Anglais
Journal information "Phytochemical Analysis" - Vol. 16, no. 5, p. 342-348 (2005)
Peer reviewed yes
Publisher JohnWiley & Sons Ltd. ((United Kingdom) Bognor Regis)
issn 0958-0344
e-issn 1099-1565
Publication status Publié
Affiliation UCL - MD/FARM - Ecole de pharmacie
MESH Subject Chromatography, Gas - methods ; Croton - chemistry ; Diterpenes - analysis - chemistry ; Molecular Structure ; Plant Leaves - chemistry ; Reproducibility of Results ; Sensitivity and Specificity
Keywords Capillary gas chromatography ; Diterpenes ; Trachylobane ; Pimarane ; Croton zambesicus ; Euphorbiaceae
Links
  1. , . 1989. Contribution aux études ethnobotaniques et floristiques en République Populaire du Bénin, Vol 1. Agence de coopération culturelle et technique: Paris.
  2. Block, Planta Med, 68, 647 (2002)
  3. Block, Phytochemistry, 65, 1165 (2004)
  4. Chapuzet, STP Pharma Pratiques, 7, 169 (1997)
  5. Fraga, Phytochem Anal, 5, 49 (1994)
  6. Hubert, Anal Chim Acta, 391, 135 (1999)
  7. Hubert, STP Pharma Pratiques, 13, 101 (2003)
  8. . 2000. Statistics for Analytical Chemistry, 5th edn. Ellis Horwood: New York.
  9. Ngadjui, Phytochemistry, 60, 345 (2002)
  10. . 1962. The Medicinal and Poisonous Plants of Southern and Eastern Africa, 2nd edn. Livingstone: London.
Bibliographic reference Block, Sébastien ; Brkic, Dejan ; Hubert, Philippe ; Quetin-Leclercq, Joëlle. A validated method for the quantification of pimarane and trachylobane diterpenes in the leaves of Croton zambesicus by capillary gas chromatography. In: Phytochemical Analysis, Vol. 16, no. 5, p. 342-348 (2005)
Permanent URL http://hdl.handle.net/2078.1/10355