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Effects of raloxifene treatment on the phenotype of blood monocytes.

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Zouaoui Boudjeltia, Karim [UCL] Durez, Patrick [UCL] Oberweis, Didier Guillaume, Michel Remacle, Claude [UCL] Gregoir, Catherine

Raloxifene (RLX), a selective oestrogen receptor modulator, has oestrogen-agonist effects on bone, lipoproteins, and homocysteine and oestrogen-antagonist activity in the breast and uterus, positioning it as a potential drug for long-term prevention of coronary heart disease in postmenopausal women. To further evaluate its influence on cardiovascular risk factors, we studied the effects of 60 mg/day RLX on serum lipid levels, inflammatory (high-sensitivity C-reactive protein, and coagulation (fibrinogen) markers, monocytes, and fibrinolysis in 15 healthy postmenopausal women. Markers were measured at baseline, after 1 month without treatment, and after 3 months of treatment. Fibrinolysis was evaluated using the euglobulin clot lysis time (ECLT) determined with a new semiautomatic optical method. Monocyte phenotype was determined by measurement of the expression of the antigens CD14, HLA-DR, and CD62-L using flow cytometry. After 3 months of RLX treatment, we observed a decrease in total cholesterol (p = 0.002), in low-density lipoprotein cholesterol (p <0.001), and in lipoprotein A (p = 0.01). Fibrinogen (p = 0.002) decreased significantly, and high-sensitivity C-reactive protein had a tendency to decrease, but this did not reach statistical significance (p = 0.06). RLX treatment had no effect on ECLT (p = 0.223) or on white blood cell, lymphocyte, and total monocyte counts (p = 0.313). Monocyte expression of HLA-DR, CD14, and CD62-L was not modified by the treatment. In conclusion, we confirm that RLX has beneficial short-term effects on levels of lipids and inflammatory markers, with no effect on fibrinolysis or monocyte phenotype.

Document type Article de périodique (Journal article) – Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
Publication date 2010
Language Anglais
Journal information "Canadian Journal of Physiology and Pharmacology" - Vol. 88, no. 5, p. 601-605 (2010)
Peer reviewed yes
Publisher N R C Research Press ((Canada) Ottawa)
issn 0008-4212
e-issn 1205-7541
Publication status Publié
Affiliations UCL - (SLuc) Service de rhumatologie
UCL - SSS/IREC/RUMA - Pôle de Pathologies rhumatismales
MESH Subject Aged ; Antigens, CD14 - biosynthesis, blood ; L-Selectin - biosynthesis, blood ; Leukocyte Count ; Lipoprotein(a) - blood ; Middle Aged ; Monocytes - drug effects, immunology, metabolism ; Postmenopause - blood, drug effects ; Raloxifene - administration & dosage, pharmacology, therapeutic use ; Selective Estrogen Receptor Modulators - administration & dosage, pharmacology, therapeutic use ; Cholesterol - blood ; Cholesterol, HDL - blood ; Coronary Disease - blood, prevention & control ; Cytokines - biosynthesis, blood ; Female ; Fibrinolysis - drug effects ; Flow Cytometry ; Humans
Links
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Bibliographic reference Zouaoui Boudjeltia, Karim ; Durez, Patrick ; Oberweis, Didier ; Guillaume, Michel ; Remacle, Claude ; et. al. Effects of raloxifene treatment on the phenotype of blood monocytes.. In: Canadian Journal of Physiology and Pharmacology, Vol. 88, no. 5, p. 601-605 (2010)
Permanent URL http://hdl.handle.net/2078.1/105552