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Nanoscale imaging of Bacillus thuringiensis flagella using atomic force microscopy

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Gillis, Annika [UCL] Dupres, Vincent [UCL] Delestrait, Guillaume [UCL] Mahillon, Jacques [UCL] Dufrêne, Yves [UCL]

Because bacterial flagella play essential roles in various processes (motility, adhesion, host interactions, secretion), studying their expression in relation to function is an important challenge. Here, we use atomic force microscopy (AFM) to gain insight into the nanoscale surface properties of two wild-type and four mutant strains of Bacillus thuringiensis exhibiting various levels of flagellation. We show that, unlike AFM in liquid, AFM in air is a simple and reliable approach to observe the morphological details of the bacteria, and to quantify the density and dimensions of their flagella. We found that the amount of flagella expressed by the six strains, as observed at the nanoscale, correlates with their microscopic swarming motility. These observations provide novel information on flagella expression in Gram-positive bacteria and demonstrate the power of AFM in genetic studies for the fast assessment of the phenotypic characteristics of bacterial strains altered in cell surface appendages.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Nanoscale" - Vol. 4, p. 1585-1591 (2012)
Peer reviewed yes
Publisher National Center for Nanoscience and Technology
issn 2040-3364
e-issn 2040-3372
Publication status Publié
Affiliations UCL - SST/ELI/ELIM - Applied Microbiology
UCL - SST/IMCN/BSMA - Bio and soft matter
Keywords Flagella ; thuringiensy ; Afm ; bacillu thuringiensy ; nanoscale
Links
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Bibliographic reference Gillis, Annika ; Dupres, Vincent ; Delestrait, Guillaume ; Mahillon, Jacques ; Dufrêne, Yves. Nanoscale imaging of Bacillus thuringiensis flagella using atomic force microscopy. In: Nanoscale, Vol. 4, p. 1585-1591 (2012)
Permanent URL http://hdl.handle.net/2078.1/107260