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Nanostructure and nanomechanics of live Phaeodactylum tricornutum morphotypes.

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Francius, Grégory [UCL] Tesson, Benoît [Nantes] Dague, Etienne [UCL] Martin-Jézéquel, Véronique [Nantes] Dufrêne, Yves [UCL]

The ultrastructure and mechanical properties of the fusiform, triradiate and ovoid morphotypes of Phaeodactylum tricornutum were investigated using atomic force microscopy. Using topographic imaging, we showed that the surface of the ovoid form is rougher than those of the two other specimens, and coated with an outer layer of extracellular polymers. Using spatially resolved force-indentation curves, we found that the valve of the ovoid form is about five times stiffer (Young modulus of approximately 500 kPa) than those of the other forms (approximately 100 kPa), a finding fully consistent with the fact that only the ovoid form has a silica valve, whereas the valves in the other two consist mostly of organic material. Notably, the girdle region of both fusiform and ovoid forms was five times softer than the valve, suggesting that this region is poor in silica and enriched in organic material. For the triradiate form, we showed the arms to be softer than the core region, presumably as a result of organelle localization. Last, we observed mucilaginous footprints of moderate stiffness (approximately 100 kPa) in the vicinity of ovoid diatoms, which we believe are secreted extracellular polymers.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2008
Journal information "Environmental microbiology" - Vol. 10, no. 5, p. 1344-56 (2008)
Peer reviewed yes
e-issn 1462-2920
issn 1462-2912
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Surface Properties ; Nanotechnology ; Nanostructures - ultrastructure ; Microscopy, Electron, Scanning ; Microscopy, Atomic Force ; Elasticity ; Diatoms - metabolism - physiology - ultrastructure ; Biomechanics
Links
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Bibliographic reference Francius, Grégory ; Tesson, Benoît ; Dague, Etienne ; Martin-Jézéquel, Véronique ; Dufrêne, Yves. Nanostructure and nanomechanics of live Phaeodactylum tricornutum morphotypes.. In: Environmental microbiology, Vol. 10, no. 5, p. 1344-56 (2008)
Permanent URL http://hdl.handle.net/2078.1/11276