De Backer, O
[UCL]
Colson, Charles
[UCL]
The genes encoding the restriction-modification system StyLTI of Salmonella typhimurium were inserted in vivo into the conjugative plasmid pULB21. This allowed us to transfer the StyLTI genes at a very high frequency and to monitor the fate of recipient cells after mating. Transfer of the StyLTI restriction and modification genes into a modificationless recipient was lethal and resulted in degradation of the cell's DNA. This indicates that, in contrast to any other known restriction-modification systems, StyLTI cannot be established after horizontal transfer into a naive host.
Bibliographic reference |
De Backer, O ; Colson, Charles. Transfer of the genes for the StyLTI restriction-modification system of Salmonella typhimurium to strains lacking modification ability results in death of the recipient cells and degradation of their DNA.. In: Journal of bacteriology, Vol. 173, no. 3, p. 1328-30 (1991) |
Permanent URL |
http://hdl.handle.net/2078.1/11389 |