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The sequence of an 8.8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase alpha-primases and bacterial GTP cyclohydrolase II.

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Skala, J. Van Dyck, L Purnelle, Bénédicte [UCL] Goffeau, André [UCL]

The DNA sequence of two contiguous 7648 bp and 1194 bp BamHI fragments from the cosmid alpha 1201 located about 60 kb from the centromere on the left arm of chromosome II from Saccharomyces cerevisiae has been determined. Sequence analysis reveals four new open reading frames longer than 300 bp: YBL0415 (309 bp), YBL0416 (4539 bp), YBL0417 (1035 bp) and YBL0414 (2115 bp), which extends into the neighbouring 5.2 kb BamHI fragment. The YBL0414 shows homologies to the mouse 68 kDa and Drosophila melanogaster 76 kDa subunits of the DNA polymerase alpha-primase complex. The YBL0417 is homologous to bacterial GTP cyclohydrolase II (EC 3.5.4.25).

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1994
Journal information "Yeast (Chichester, England)" - Vol. 10 Suppl A, p. S13-24 (1994)
Peer reviewed yes
issn 0749-503X
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Amino Acid Sequence ; Animals ; Base Sequence ; Chromosomes, Fungal ; DNA Primase ; GTP Cyclohydrolase - genetics ; Genes, Fungal - genetics ; Molecular Sequence Data ; Open Reading Frames - genetics ; RNA Nucleotidyltransferases - genetics ; Restriction Mapping ; Saccharomyces cerevisiae - genetics ; Sequence Alignment ; Sequence Homology, Amino Acid
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Bibliographic reference Skala, J. ; Van Dyck, L ; Purnelle, Bénédicte ; Goffeau, André. The sequence of an 8.8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase alpha-primases and bacterial GTP cyclohydrolase II.. In: Yeast (Chichester, England), Vol. 10 Suppl A, p. S13-24 (1994)
Permanent URL http://hdl.handle.net/2078.1/12005