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SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.

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Wattiau, Pierre [UCL] Cornelis, Guy

Pathogenic yersiniae secrete a set of 11 anti-host proteins called Yops. The yop genes, scattered around the pYV plasmid, constitute a thermoinduced regulon controlled by the product of virF gene. The secretion of the Yops also requires the presence of the products of the other vir genes and operons, namely virA, virB and virC. The large virC operon and presumably some genes of the virA region encode a new secretion system. Mutations in any of these vir genes impair the production of all the Yops. In contrast, mutations in the yerA locus, located close to yopE, specifically abolish the expression of the cytotoxin YopE. We describe here the counterpart of yerA in Yersinia enterocolitica W22703. We demonstrate that the gene product of yerA regulates the production of YopE at a post-transcriptional level. It specifically binds the YopE protein. We consider that it acts as a specific chaperone and we call it SycE (for specific YopE chaperone). We hypothesize that SycE is a link between translation and the specific Yop export machinery. It is the first representative of a new family of pYV-encoded proteins.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1993
Journal information "Molecular microbiology" - Vol. 8, no. 1, p. 123-31 (1993)
Peer reviewed yes
issn 0950-382X
Publication status Publié
Affiliation UCL
MESH Subject Bacterial Outer Membrane Proteins - biosynthesis - secretion ; Bacterial Proteins - genetics - metabolism ; Base Sequence ; Cytoplasm - chemistry ; Molecular Sequence Data ; Mutagenesis ; Protein Binding ; Recombinant Fusion Proteins - metabolism ; Trans-Activators - genetics ; Transcription, Genetic ; Yersinia enterocolitica - genetics - physiology
Links
  1. Biot T., J Gen Microbiol, 134, 1525 (1988)
  2. Bochkareva E. S., Lissin N. M., Girshovich A. S., Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein, 10.1038/336254a0
  3. Bodeus M., Rat Hybridoma and Rat Monoclonal Antibodies, 335 (1990)
  4. Bolin I., Infect Immun, 43, 72 (1984)
  5. Brissette Janice L., Russel Marjorie, Secretion and membrane integration of a filamentous phage-encoded morphogenetic protein, 10.1016/0022-2836(90)90266-o
  6. China B., Mol Microbiol, 4, 1585 (1990)
  7. Cornelis G., Molecular Biology of Bacterial Infections. SGM Symposium Series 49, 231 (1992)
  8. Cornelis G., Colson C., Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli, 10.1099/00221287-87-2-285
  9. Cornelis Guy, Sory M.P., Laroche Y., Derclaye I., Genetic analysis of the plasmid region controlling virulence in Yersinia enterocolitica 0:9 by Mini-Mu insertions and lac gene fusions, 10.1016/0882-4010(86)90067-7
  10. Cornelis Guy, Vanootegem J.-C., Sluiters C., Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes, 10.1016/0882-4010(87)90078-7
  11. Cornelis G., J Bacteriol, 171, 254 (1989)
  12. Crooke E., EMBO J, 7, 1831 (1988)
  13. Dale R.M.K., McClure B.A., Houchins J.P., A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18 S rDNA, 10.1016/0147-619x(85)90053-8
  14. Cock H., Mol Microbiol, 6, 599 (1992)
  15. D'Enfert C., J Biol Chem, 264, 17462 (1989)
  16. Eisenberg D., Schwarz E., Komaromy M., Wall R., Analysis of membrane and surface protein sequences with the hydrophobic moment plot, 10.1016/0022-2836(84)90309-7
  17. Forsberg A., J Bacteriol, 172, 1547 (1990)
  18. Forsberg A., Mol Microbiol, 5, 977 (1991)
  19. Hartl Franz-Ulrich, Lecker Stewart, Schiebel Elmar, Hendrick Joseph P., Wickner William, The binding cascade of SecB to SecA to SecYE mediates preprotein targeting to the E. coli plasma membrane, 10.1016/0092-8674(90)90160-g
  20. Kaniga Koné, Delor Isabelle, Cornelis Guy R., A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica, 10.1016/0378-1119(91)90599-7
  21. Kumamoto C.A., Proc Natl Acad Sci USA, 86, 5320 (1989)
  22. Kumamoto C.A., Mol Microbiol, 5, 19 (1991)
  23. Lambert de Rouvroit C., Mol Microbiol, 6, 395 (1992)
  24. Michiels T., J Bacteriol, 173, 1677 (1991)
  25. Michiels T., Infect Immun, 58, 2840 (1990)
  26. Michiels T., J Bacteriol, 173, 4994 (1991)
  27. J.H. Miller (1972 ) InExperiments in Molecular Genetics . Cold Spring Harbor, New York: Cold Spring Harbor Labortory Press, pp.352 -355 .
  28. Miller V.L., J Bacteriol, 170, 2575 (1988)
  29. Mulder B., Infect Immun, 57, 2534 (1989)
  30. Neu H.C., J Bacteriol, 94, 1934 (1967)
  31. Plano G.V., J Bacteriol, 173, 7293 (1991)
  32. Portnoy D.A., J Bacteriol, 148, 877 (1981)
  33. Skurnik M., Mol Microbiol, 5, 585 (1991)
  34. Sory M.-P., Cornelis G., Yersinia enterocolitica O:9 as a potential live oral carrier for protective antigens, 10.1016/0882-4010(88)90028-9
  35. Tabor S., Richardson C. C., A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes., 10.1073/pnas.82.4.1074
  36. Viitanen A.-M., J Bacteriol, 172, 3152 (1990)
  37. Heijne G., Nucl Acids Res, 14, 4683 (1986)
Bibliographic reference Wattiau, Pierre ; Cornelis, Guy. SycE, a chaperone-like protein of Yersinia enterocolitica involved in Ohe secretion of YopE.. In: Molecular microbiology, Vol. 8, no. 1, p. 123-31 (1993)
Permanent URL http://hdl.handle.net/2078.1/12131