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Co-culture of two-cell rat embryos on cell monolayers.

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Pampfer, Serge [UCL] Vanderheyden, Ivo [UCL] Michiels, B. [UCL] De Hertogh, René [UCL]

Monolayers of 2 different populations of uterine cells and of fetal fibroblasts were evaluated for the support of rat embryo development in vitro. Compared to controls, cultures performed in Earle's buffered saline solution (EBSS) alone, the cleavage rate of 2-cell embryos to the 4-cell stage was significantly increased when the embryos were cocultured for 24 h with mixed uterine stromal and myometrial cells (70.7 vs. 56.0%; P less than 0.01). Coculture of 2-cell embryos with either uterine epithelial-stromal or stromal-myometrial cells in medium TC 199 (M199) for 24 h significantly increased the cleavage rate to the 4-cell stage compared to controls in the same medium (respectively, 78.3 and 77.6 vs. 49.9%; P less than 0.01). The development was not improved when fibroblasts were used as feeder cells. After 48 h, the proportion of 4-cell embryos showing cellular fragmentation was significantly decreased in the presence of either epithelial-stromal or stromal-myometrial cells in M199 compared to controls (respectively, 18.4 and 20.0 vs. 43.8%; P less than 0.01). Coculture in EBSS or with fibroblasts failed to prevent embryo degeneration. In one coculture with stromal-epithelial cells in M199, 6/11 embryos proceeded beyond the 4-cell stage, two of them reaching the 8-cell stage. No embryo developed beyond that stage in our study. Although considerable efforts remain necessary to achieve further growth, these results suggest that coculture offers promise as a means of supporting the in vitro development of rat embryos.

Document type Article de périodique (Journal article) – In Vitro, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1990
Journal information "In vitro cellular & developmental biology : journal of the Tissue Culture Association" - Vol. 26, no. 10, p. 944-8 (1990)
issn 0883-8364
Publication status Publié
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Animals ; Cell Division ; Cells, Cultured ; Cleavage Stage, Ovum - cytology ; Culture Media ; Ectogenesis ; Female ; Fibroblasts - cytology ; Mitosis ; Rats ; Rats, Inbred Strains - embryology ; Uterus - cytology
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  1. Abramczuk, J.; Solter, D.; Koprowski, H. The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61:378–383; 1977.
  2. Allen R. L., Wright R. W., In Vitro Development of Porcine Embryos in Coculture with Endometrial Cell Monolayers or Culture Supernatants, 10.2527/jas1984.5961657x
  3. Bavister Barry D., Studies on the Developmental Blocks in Cultured Hamster Embryos, The Mammalian Preimplantation Embryo (1987) ISBN:9781468453348 p.219-249, 10.1007/978-1-4684-5332-4_11
  4. Bavister, B. D.; Leibfried, M.; Lieberman, G. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol. Reprod. 28:235–247; 1983.
  5. Bell, S. C.; Searle, R. F. Differentiation of decidual cells in mouse endometrial cell cultures. J. Reprod. Fertil. 61:425–433; 1981.
  6. Bongso, A.; Soon-chye, Ng; Sathananthan, H., et al. Improved quality of human embryos when co-cultured with human ampullary cells. Human Reprod. 4:706–713; 1989.
  7. Chatot, C. L.; Ziomek, C. A.; Bavister, B. D., et al. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J. Reprod. Fertil. 86:679–688; 1989.
  8. Cole, R. J.; Paul, J. Properties of cultured preimplantation mouse and rabbit embryos and cell strains derived from them. In: Wolstenholm, G. E. M.; O'Connor, M., eds. Preimplantation stages of pregnancy. Boston: Little, Brown & Company; 1965:82–112.
  9. Cross, P. C.; Brinster, R. L. The sensitivity of one-cell mouse embryos to pyruvate and lactate. Exp. Cell Res. 77:57–62; 1973.
  10. Davis, D. L. Culture and storage of pig embryos. J. Reprod. Fertil. Suppl. 33:115–124; 1985.
  11. Ebert, K. M.; Hammer, R. E.; Papaioannou, V. E. A simple method for counting nuclei in the preimplantation mouse embryo. Experientia 41:1207–1209; 1985.
  12. Farrell, P. S.; Bavister, B. D. Short-term exposure of two-cell hamster embryos to collection media is detrimental to viability. Biol. Reprod. 31:109–114; 1984.
  13. Gandolfi, F.; Moor, R. M. Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J. Reprod. Fertil. 81:23–28; 1987.
  14. Glasser Stanley R., McCormack Shirley A., Separated Cell Types as Analytical Tools in the Study of Decidualization and Implantation, Cellular and Molecular Aspects of Implantation (1981) ISBN:9781461331827 p.217-239, 10.1007/978-1-4613-3180-3_16
  15. Goddard, M. J.; Pratt, H. P. M. Control of events during early cleavage of the mouse embryo: an analysis of the 2-cell block. J. Embryol. Exp. Morphol. 73:111–133; 1983.
  16. Goto, K.; Kajihara, Y.; Kosaka, S., et al. Pregnancies after coculture of cumulus cells with bovine embryos derived from in vitro fertilization of in vitro matured follicular oocytes. J. Reprod. Fertil. 83:753–758; 1988.
  17. Kuzan Frank B., Wright Raymond W., Observations on the Development of Bovine Morulae on Various Cellular and Noncellular Substrata, 10.2527/jas1982.544811x
  18. Kuzan, F. B.; Wright, R. W. Blastocyst expansion, hatching and attachment of porcine embryos co-cultured with bovine fibroblasts in vitro. Anim. Reprod. Sci. 5:57–63; 1982.
  19. Loutradis, D.; John, D.; Kiessling, A. A. Hypoxanthine causes a 2-cell block in random-bred mouse embryos. Biol. Reprod. 37:311–316; 1987.
  20. McCormack, S.; Glasser, S. R. Differential response of individual uterine cell types from immature rats treated with estradiol. Endocrinology 106:1634–1649; 1980.
  21. Pampfer, S.; Streffer, C. Increased chromosome aberration level in cells from mouse fetuses after zygote X-irradiation. Int. J. Radiat. Biol. 55:85–92; 1989.
  22. Quinn, P.; Harlow, G. M. The effect of oxygen on the development of preimplantation mouse embryos in vitro. J. Exp. Zool. 206:73–80; 1978.
  23. Rexroad C. E., Powell A. M., Co-Culture of Ovine Ova with Oviductal Cells in Medium 199, 10.2527/jas1988.664947x
  24. Schini, S. A.; Bavister, B. D. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol. Reprod. 39:1183–1192; 1988.
  25. Tarkowski, A. K. An air-drying method for chromosome preparation from mouse eggs. Cytogenetics 5:394–400; 1966.
  26. Thibault, C. La culture in vitro de l'oeuf de vache. Ann. Biol. Anim. Biochem. Biophys. 6:159–164; 1966.
  27. Wiemer, K. E.; Denniston, R. S.; Amborski, G. F., et al. A fetal bovine uterine fibroblast monolayer system for in vitro culture of bovine embryos. J. Anim. Sci. Suppl. 1:53–65; 1987.
  28. Wiemer, K.; Casey, P.; Godke, R. The culture of equine embryos using a new fetal uterine monolayer culture system. Theriogenology 29:327–332; 1988.
  29. Wiemer, K. E.; Cohen, J.; Ambroski, G. F., et al. In vitro development and implantation of human embryos following culture on fetal bovine uterine fibroblast cells. Hum. Reprod. 4:595–600; 1989.
  30. Wiemer Klaus E., Cohen Jacques, Wiker Sharon R., Malter Henry E., Wright Graham, Godke Robert A., Coculture of human zygotes on fetal bovine uterine fibroblasts: embryonic morphology and implantation*, 10.1016/s0015-0282(16)60926-3
  31. Wittingham, D. G. Fertilization, early development and storage of mammalian ova in vitro. In: Balls, M.; Wild, A. E., eds. Early development of mammals. Cambridge, UK: Cambridge University Press; 1966:63–149.
  32. White, K. L.; Hehnke, K.; Richards, L. F., et al. Early embryonic development in vitro by co-culture with oviductal epithelial cells in pig. Biol. Reprod. 41:425–430; 1989.
  33. Wright Raymond W., O’Fallon James V., Growth of Domesticated Animal Embryos in Vitro, The Mammalian Preimplantation Embryo (1987) ISBN:9781468453348 p.251-271, 10.1007/978-1-4684-5332-4_12
Bibliographic reference Pampfer, Serge ; Vanderheyden, Ivo ; Michiels, B. ; De Hertogh, René. Co-culture of two-cell rat embryos on cell monolayers.. In: In vitro cellular & developmental biology : journal of the Tissue Culture Association, Vol. 26, no. 10, p. 944-8 (1990)
Permanent URL http://hdl.handle.net/2078.1/13774