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Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

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Sengottaiyan, Palanivelu [UCL] Petrlova, Jitka [Department of Experimental Medical Science, Lund University, Sweden] Lagerstedt, Jens O. [Department of Experimental Medical Science, Lund University, Sweden] Ruiz-Pavon, Lorena [Linnaeus University Centre for Biomaterials Chemistry, Department of Chemistry and Biomedical Sciences, Linnaeus University, Sweden] Budamagunta, Madhu S. [Department of Biochemistry and Molecular Medicine, University of California, Davis, USA] Persson, Bengt L. [Linnaeus University Centre for Biomaterials Chemistry, Department of Chemistry and Biomedical Sciences, Linnaeus University, Sweden]

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na+/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate. © 2013 The Authors.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "Biochemical and Biophysical Research Communications" - Vol. 436, no. 3, p. 551-556 (2013)
Peer reviewed yes
Publisher Elsevier Inc.
e-issn 1090-2104
issn 0006-291X
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
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Bibliographic reference Sengottaiyan, Palanivelu ; Petrlova, Jitka ; Lagerstedt, Jens O. ; Ruiz-Pavon, Lorena ; Budamagunta, Madhu S. ; et. al. Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89. In: Biochemical and Biophysical Research Communications, Vol. 436, no. 3, p. 551-556 (2013)
Permanent URL http://hdl.handle.net/2078.1/160649