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Nanoscale adhesion forces between the fungal pathogen Candida albicans and macrophages

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El-Kirat-Chatel, Sofiane Dufrêne, Yves [UCL]

The development of fungal infections is tightly controlled by the interaction of fungal pathogens with host immune cells. While the recognition of specific fungal cell wall components by immune receptors has been widely investigated, the molecular forces involved are not known. In this Communication, we show the ability of single-cell force spectroscopy to quantify the specific adhesion forces between the fungal pathogen Candida albicans and macrophages. The Candida–macrophage adhesion force is strong, up to B3000 pN, and corresponds to multiple cumulative bonds between lectin receptors expressed on the macrophage membrane and mannan carbohydrates on the fungal cell surface. Adhesion force signatures show constant force plateaus, up to 4100 lm long, reflecting the extraction of elongated tethers from the macrophage membrane, a phenomenon which may increase the duration of intercellular adhesion. Adhesion strengthens with time, suggesting that the macrophage membrane engulfs the pathogen quickly after initial contact, leading to its internalization. The force nanoscopy method developed here holds great promise for understanding and controlling the early stages of microbe–immune interactions.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2016
Language Anglais
Journal information "Nanoscale Horizons : the home for rapid reports of exceptional significance in nanoscience and nanotechnology" - Vol. 1, no.1, p. 69-74 (2016)
Peer reviewed yes
Publisher Royal Society of Chemistry
issn 2055-6756
e-issn 2055-6764
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
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Bibliographic reference El-Kirat-Chatel, Sofiane ; Dufrêne, Yves. Nanoscale adhesion forces between the fungal pathogen Candida albicans and macrophages. In: Nanoscale Horizons : the home for rapid reports of exceptional significance in nanoscience and nanotechnology, Vol. 1, no.1, p. 69-74 (2016)
Permanent URL http://hdl.handle.net/2078.1/168766