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Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith

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van den Biggelaar, Ludivine [UCL] Soumillion, Patrice [UCL] Debecker, Damien P. [UCL]

ω-Transaminases have been immobilized on macrocellular silica monoliths and used as heterogeneous biocatalysts in a continuous flow mode enantioselective transamination reaction. The support was prepared by a sol-gel method based on emulsion templating. The enzyme was immobilized on the structured silica monoliths both by adsorption, and by covalent grafting using amino-functionalized silica monoliths and glutaraldehyde as a coupling agent. A simple reactor set-up based on the use of a heat-shrinkable Teflon tube is presented and successfully used for the continuous flow kinetic resolution of a chiral amine, 4-bromo-α-methylbenzylamine. The porous structure of the supports ensures effective mass transfer and the reactor works in the plug flow regime without preferential flow paths. When immobilized in the monolith and used in the flow reactor, transaminases retain their activity and their enantioselectivity. The solid biocatalyst is also shown to be stable both on stream and during storage. These essential features pave the way to the successful development of an environmentally friendly process for chiral amines production.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2017
Language Anglais
Journal information "Catalysts" - Vol. 7, no.54, p. 1-13 (2017)
Peer reviewed yes
Publisher M D P I AG ((Switzerland) Basel)
issn 2073-4344
e-issn 2073-4344
Publication status Publié
Affiliations UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity
UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Keywords chiral amines ; biocatalysis ; silica monolith ; enzyme immobilization ; flow chemistry
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Bibliographic reference van den Biggelaar, Ludivine ; Soumillion, Patrice ; Debecker, Damien P.. Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith. In: Catalysts, Vol. 7, no.54, p. 1-13 (2017)
Permanent URL http://hdl.handle.net/2078.1/182359