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Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality

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Vermeulen, Maxime [UCL] Del Vento, Federico [UCL] de Michele, Francesca [UCL] Poels, Jonathan [UCL] Wyns, Christine [UCL]

Cryopreservation of immature testicular tissue before chemo/radiotherapy is the only option to preserve fertility of cancer-affected prepubertal boys. To avoid reintroduction of malignant cells, development of a transplantable scaffold by decellularization of pig immature testicular tissue (ITT) able to support decontaminated testicular cells could be an option for fertility restoration in these patients. We, therefore, compared decellularization protocols to produce a cytocompatible scaffold. Fragments of ITT from 15 piglets were decellularized using three protocols: sodium dodecyl sulfate (SDS)-Triton (ST), Triton-SDS-Triton (TST) and trypsin 0.05%/ethylenediaminetetraacetic acid (EDTA) 0.02%-Triton (TET) with varying detergent concentrations. All protocols were able to lower DNA levels. Collagen retention was demonstrated in all groups except ST 1%, and a significant decrease in glycosaminoglycans was observed in the TST 1% and TET 1% groups. When Sertoli cells (SCs) were cultured with decellularized tissue, no signs of cytotoxicity were detected. A higher SC proliferation rate and greater stem cell factor secretion were observed than with SCs cultured without scaffold. ST 0.01% and TET 3% conditions offered the best compromise in terms of DNA elimination and extracellular matrix (ECM) preservation, while ensuring good attachment, proliferation and functionality of human SCs. This study demonstrates the potential of using decellularized pig ITT for human testicular tissue engineering purposes.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "International Journal of Molecular Sciences" - Vol. 19, no. 1, p. 227 [1-15] (2018)
Peer reviewed yes
Publisher MDPI AG ((Switzerland) Basel)
e-issn 1422-0067
Publication status Publié
Affiliations UCL - SSS/IREC/GYNE - Pôle de Gynécologie
UCL - (SLuc) Service de gynécologie et d'andrologie
Keywords Decellularization ; Decellularized tissue ; Extracellular matrix ; Fertility preservation ; Immature testicular tissue ; Regenerative medicine ; Scaffold ; Testicular organoid ; Tissue engineering
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Bibliographic reference Vermeulen, Maxime ; Del Vento, Federico ; de Michele, Francesca ; Poels, Jonathan ; Wyns, Christine. Development of a Cytocompatible Scaffold from Pig Immature Testicular Tissue Allowing Human Sertoli Cell Attachment, Proliferation and Functionality. In: International Journal of Molecular Sciences, Vol. 19, no. 1, p. 227 [1-15] (2018)
Permanent URL http://hdl.handle.net/2078.1/193340