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Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities

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Loján, Paul [UCL] Senés-Guerrero, Carolina [Ludwig-Maximilians University Munich] Suárez, Juan Pablo [Universidad Técnica Particular de Loja] Kromann, Peter [International Potato Center (CIP)] Schüßler, Arthur [Ludwig-Maximilians University Munich] Declerck, Stephan [UCL]

A field trial was conducted in two localities of the Ecuadorian Andes to evaluate potato (Solanum tuberosum c.v. INIAP - Fripapa) response to inoculation with four commercial products containing the arbuscular mycorrhizal fungus (AMF) strain Rhizophagus irregularis DAOM 197198. In parallel, potato roots were analysed using 454 GS-FLX+ sequencing of c. 800 bp of the nuclear LSU rRNA gene to assess the associated AMF communities. To evaluate inoculation success, sequence reads of R. irregularis on the root samples were compared between inoculated and not inoculated plants by analysing the frequency of occurrence (FO) and relative read abundance (RA). None of the commercial products significantly increased potato yield. Instead, the AMF communities were dominated by an unknown Acaulospora sp. (Sp14) found at high FO and RA in both localities. Rhizophagus irregularis was found in most of the roots of both inoculated and not inoculated plants at both localities. However, its abundance was unexpectedly low indicating poor inoculum establishment. Clearly, many factors have to be taken in consideration for the successful application of AMF-based inoculants. For the Ecuadorian field trials, several causes may explain the lack or poor establishment of R. irregularis such as inoculation technique, agricultural practices, biotic and abiotic conditions and competition with native AMF species.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2017
Language Anglais
Journal information "Symbiosis" - Vol. 73, no. 1, p. 45-56 (2016)
Peer reviewed yes
Publisher Springer Netherlands (Dordrecht)
issn 0334-5114
e-issn 1878-7665
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Arbuscular mycorrhizal fungi ; Commercialinoculum ; LSU rRNA gene ; Rhizophagus irregularis ; Solanum tuberosum
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Bibliographic reference Loján, Paul ; Senés-Guerrero, Carolina ; Suárez, Juan Pablo ; Kromann, Peter ; Schüßler, Arthur ; et. al. Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities. In: Symbiosis, Vol. 73, no. 1, p. 45-56 (2016)
Permanent URL http://hdl.handle.net/2078.1/203889