Osteogenic Differentiation of Adipose-Derived Stromal Cells: From Bench to Clinics

Kuterbekov, Mirasbek [UCL] Jonas, Alain M. [UCL] Glinel, Karine [UCL] Picart, Catherine [CNRS, LMGP, Grenoble, France; Grenoble Institute of Technology, University Grenoble Alpes, LMGP, Grenoble, France; CEA, Institute of Interdisciplinary Research of Grenoble (IRIG), Biomimetism and Regenerative Medicine Lab, Université Grenoble-Alpes/CEA/CNRS]

In addition to mesenchymal stem cells, adipose derived stem/stromal cells are an attractive source for a large variety of cell-based therapies. One of their most important potential applications is related to the regeneration of bone tissue thanks to their capacity to differentiate in bone cells. However, this requires a proper control of their osteogenic differentiation with depends on the initial characteristics of harvested cells but also on the conditions used for their culture. In this review, we first briefly describe the pre-clinical and clinical trials using ASCs for bone regeneration and present the quantitative parameters used to characterize the osteogenic differentiation of ASCs. We then focus on the soluble factors influencing the osteogenic differentiation of ACS, including the steroid hormones and various growth factors, notably the most osteoinductive ones, the bone morphogenetic proteins (BMPs).