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Chronic relapsing experimental allergic encephalomyelitis (CREAE) in plasminogen activator inhibitor-1 knockout mice: the effect of fibrinolysis during neuroinflammation

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East, E. [UCL] Gveric, D. [UCL] Baker, D. [UCL] Pryce, G. [UCL] Lijnen, H. R. [UCL] Cuzner, M. L. [UCL]

During neuroinflammation in multiple sclerosis (MS) fibrinogen, not normally present in the brain or spinal cord, enters the central nervous system through a compromised blood-brain barrier. Fibrin deposited on axons is ineffectively removed by tissue plasminogen activator (tPA), a key contributory factor being the upregulation of plasminogen activator inhibitor-1 (PAI-1). Aims: This study investigated the role of PAI-1 during experimental neuroinflammatory disease. Methods: Chronic relapsing experimental allergic encephalomyelitis (CREAE), a model of MS, was induced with spinal cord homogenate in PAI-1 knockout (PAI-1(-/-)) and wild type (WT) mice, backcrossed onto the Biozzi background. Results: Disease incidence and clinical severity were reduced in PAI-1(-/-) mice, with animals developing clinical signs significantly later than WTs. Clinical relapses were absent in PAI-1(-/-) mice and the subsequent reduction in neuroinflammation was coupled with a higher capacity for fibrinolysis in spinal cord samples from PAI-1(-/-) mice, in association with increased tPA activity. Axonal damage was less apparent in PAI-1(-/-) mice than in WTs, implicating fibrin in both inflammatory and degenerative events during CREAE. Conclusions: PAI-1 is a potential target for therapy in neuroinflammatory degenerative diseases, allowing effective fibrin removal and potentially reducing relapse rate and axonal damage.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2008
Language Anglais
Journal information "Neuropathology and Applied Neurobiology" - Vol. 34, no. 2, p. 216-230 (2008)
Peer reviewed yes
Publisher Blackwell Publishing (Oxford)
issn 0305-1846
e-issn 1365-2990
Publication status Publié
Affiliation UCL
Keywords experimental allergic encephalomyelitis ; fibrinolysis ; multiple sclerosis ; plasminogen activator inhibitor-1 ; plasminogen
Links
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Bibliographic reference East, E. ; Gveric, D. ; Baker, D. ; Pryce, G. ; Lijnen, H. R. ; et. al. Chronic relapsing experimental allergic encephalomyelitis (CREAE) in plasminogen activator inhibitor-1 knockout mice: the effect of fibrinolysis during neuroinflammation. In: Neuropathology and Applied Neurobiology, Vol. 34, no. 2, p. 216-230 (2008)
Permanent URL http://hdl.handle.net/2078.1/36771