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Monoclonal antibody 57B stains tumor tissues that express gene MAGE-A4

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Landry, C Marbaix, Etienne [UCL] Brasseur, Francis [UCL] Spagnoli, GC Boon, Thierry [UCL] Coulie, Pierre G. [UCL] Godelaine, D.

Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MACE A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4. (C) 2000 Wiley-Liss, Inc.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2000
Language Anglais
Journal information "International Journal of Cancer" - Vol. 86, no. 6, p. 835-841 (2000)
Peer reviewed yes
Publisher Wiley-liss (New York)
issn 0020-7136
e-issn 1097-0215
Publication status Publié
Affiliations UCL - Cliniques universitaires Saint-Luc
UCL - MD/MNOP - Département de morphologie normale et pathologique
UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Unité d'oncologie médicale
UCL - (SLuc) Service d'anatomie pathologique
UCL - MD/BICL - Département de biochimie et de biologie cellulaire
UCL - (SLuc) Service d'hépato-gastro-entérologie
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Bibliographic reference Landry, C ; Marbaix, Etienne ; Brasseur, Francis ; Spagnoli, GC ; Boon, Thierry ; et. al. Monoclonal antibody 57B stains tumor tissues that express gene MAGE-A4. In: International Journal of Cancer, Vol. 86, no. 6, p. 835-841 (2000)
Permanent URL http://hdl.handle.net/2078.1/43520