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The minimal fusion peptide of simian immunodeficiency virus corresponds to the 11 first residues of gp32

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Lorin, Aurelien Lins, Laurence Stroobant, Vincent [UCL] Brasseur, Robert Charloteaux, Benoit

We had previously predicted successfully the minimal fusion peptides (FPs) of the human immunodeficiency virus 1 (HIV-1) gp41 and the bovine leukemia virus (BLV) gp30 using an original approach based on the obliquity/fusogenicity relationship of tilted peptides. In this paper, we have used the same method to predict the shortest FP capable of inducing optimal fusion in vitro of the simian immunodeficiency virus (SRT) mac isolate and of other SIVs and human immunodeficiency virus (HIV-2) isolates. In each case, the 11-residue-long peptide was predicted as the minimal FP. For the SfV mac isolate, liposome lipid-mixing and leakage assays confirmed that this peptide is the shortest peptide inducing optimal fusion in vitro, being therefore the minimal FP. These results are another piece of evidence that the tilted properties of FPs are important for the fusion process and that our method can be used to predict the minimal FPs of other viruses. Copyright (c) 2007 European Peptide Society and John Wiley & Sons, Ltd.

Document type Communication à un colloque (Conference Paper) – Présentation orale avec comité de sélection
Access type Accès restreint
Publication date 2008
Language Anglais
Conference "2nd Workshop on Biophysics of Membrane-Active Peptides", Lisbon(Portugal) (2007)
Journal information "Journal of Peptide Science" - Vol. 14, no. 4, p. 423-428 (2008)
Peer reviewed yes
issn 1075-2617
e-issn 1099-1387
Publisher John Wiley & Sons Ltd (Chichester)
Affiliation UCL - Autre
Keywords Membrane Fusion ; Fusion Peptide ; Tilted Peptide ; Molecular Modelling ; Hiv-1 ; Hiv-2 ; Lipid Mixing ; Leakage
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Bibliographic reference Lorin, Aurelien ; Lins, Laurence ; Stroobant, Vincent ; Brasseur, Robert ; Charloteaux, Benoit. The minimal fusion peptide of simian immunodeficiency virus corresponds to the 11 first residues of gp32.2nd Workshop on Biophysics of Membrane-Active Peptides (Lisbon(Portugal), 2007). In: Journal of Peptide Science, Vol. 14, no. 4, p. 423-428 (2008)
Permanent URL http://hdl.handle.net/2078.1/59296