User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Lung permeability, antioxidant status, and NO2 inhalation: A selenium supplementation study in rats

Primary tabs

De Burbure, Claire [UCL] Heilier, Jean-François [UCL] Nève, J. Becker, A. Albrecht, C. Bernard, Alfred [UCL]

Little is known about antioxidant status, selenium status in particular, and lung response to NO2, which acts as a proinflammatory air pollutant. The effects of a low selenium diet (1.3 mu g Se/d) with or without selenium supplementation were therefore studied in 128 Wistar rats, 2 mo old, male exposed to either acute (50 ppm, 30 min), intermittent subacute (5 ppm, 6 h/d, 5 d), intermittent long-term NO2 (1 ppm, 10 ppm, 6 h/d, 5 d/wk, 28 d), or normal atmospheric air (controls). Following sacrifice, measurements of lipid peroxidation ( thiobarbituric acid-reactive substances, chemiluminescence), antioxidative protective enzymes (glutathione peroxidase [GPx], superoxide dismutase [SOD], glutathione S-transferase [GST], ceruloplasmin), lung damage ( lactate dehydrogenase, alkaline and acid phosphatases), lung permeability ( total protein, albumin), and inflammation (cell populations), along with the determination of new biomarkers such as CC16 (Clara-cell protein), were performed in serum and bronchoalveolar lavage fluid (BALF). While selenium-supplemented animals had increased GPx activity in serum prior to inhalation experiments, they also had decreased BALF CC16, blood SOD, and GST levels. Nevertheless, the protective role of normal selenium status with respect to NO2 lung toxicity was evident both for long-term and acute exposures, as the increase in BALF total proteins and corresponding decrease in serum (indicating increased lung permeability) was significantly more pronounced in selenium-deficient animals. During the various inhalation experiments, serum CC16 demonstrated its key role as an early marker of increased lung permeability. These findings corroborate the important role of selenium status in NO2 oxidative damage modulation, but also indicate, in view of its negative impact on CC16, a natural anti-inflammatory and immunosuppressor, that caution should be used prior to advocating selenium supplementation.

Document type Communication à un colloque (Conference Paper) – Présentation orale avec comité de sélection
Access type Accès restreint
Publication date 2007
Language Anglais
Conference "Conference on Strategies for Clean Air and Health", Rome(Italy) (Nov 05-07, 2003)
Journal information "Journal of Toxicology and Environmental Health. Part A" - Vol. 70, no. 3-4, p. 284-294 (2007)
Peer reviewed yes
issn 1528-7394
e-issn 1087-2620
Publisher Taylor & Francis Inc (Philadelphia)
Affiliation UCL - MD/ESP - Ecole de santé publique
Links
  1. Baker Cathy S., Evans Timothy W., Haslam Patricia L., Measurement of Ceruloplasmin in the Lungs of Patients with Acute Respiratory Distress Syndrome: Is Plasma or Local Production the Major Source?, 10.1159/000067469
  2. Barth Peter J., Müller Bernd, Effects of Nitrogen Dioxide Exposure on Clara Cell Proliferation and Morphology, 10.1016/s0344-0338(99)80052-1
  3. Beauchamp Charles, Fridovich Irwin, Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels, 10.1016/0003-2697(71)90370-8
  4. Beilstein M.A., Whanger P.D., Glutathione peroxidase activity and chemical forms of selenium in tissues of rats given selenite or selenomethionine, 10.1016/0162-0134(88)80032-1
  5. Bernard A., Eur. Respir. J., 5, 1231 (1992)
  6. Blomberg A., Am. J. Respir. Crit Care Med., 159, 536 (1999)
  7. Broeckaert Fabrice, Arsalane Karim, Hermans Cédric, Bergamaschi Enrico, Brustolin Angelita, Mutti Antonio, Bernard Alfred, Serum Clara Cell Protein: A Sensitive Biomarker of Increased Lung Epithelium Permeability Caused by Ambient Ozone, 10.1289/ehp.00108533
  8. Chang Ling-Yi, Graham Judith A., Miller Frederick J., Ospital Jean J., Crapo James D., Effects of subchronic inhalation of low concentrations of nitrogen dioxide, 10.1016/0041-008x(86)90321-2
  9. Chapin K., Manual of clinical microbiology, 33 (1995)
  10. Chen Hao, Tappel Al L., Vitamin E, Selenium, Trolox C, Ascorbic Acid Palmitate, Acetylcysteine, Coenzyme Q, β-Carotene, Canthaxanthin, and (+)-Catechin Protect Against Oxidative Damage to Kidney, Heart, Lung and Spleen, 10.3109/10715769509147537
  11. Dierynck I, Bernard A, Roels H, De Ley M, Potent inhibition of both human interferon-gamma production and biologic activity by the Clara cell protein CC16., 10.1165/ajrcmb.12.2.7865218
  12. Dillard Cora J., Sagai Masaru, Tappel Al.L., Respiratory pentane: A measure of in vivo lipid peroxidation applied to rats fed diets varying in polyunsaturated fats, vitamin E, and selenium and exposed to nitrogen dioxide, 10.1016/0378-4274(80)90128-9
  13. Dixon W. J., Analysis of Extreme Values, 10.1214/aoms/1177729747
  14. Dixon W. J., Ratios Involving Extreme Values, 10.1214/aoms/1177729693
  15. Dogra S. C., Acta Vitaminol. Enzymol., 5, 47 (1983)
  16. Giulivi C, Lung damage in paraquat poisoning and hyperbaric oxygen exposure: Superoxide-mediated inhibition of phospholipase a2, 10.1016/0891-5849(94)00111-v
  17. Gray E. L., AMA. Arch. Ind. Health., 10, 418 (1954)
  18. Guo L., Biomed. Environ. Sci., 14, 241 (2001)
  19. Habig W. H., J. Biol. Chem., 249, 7130 (1974)
  20. Halatek T., Eur. Respir. J., 11, 726 (1998)
  21. Hatch Gary E., Slade Ralph, Selgrade MaryJane K., Stead Andrew G., Nitrogen dioxide exposure and lung antioxidants in ascorbic acid-deficient guinea pigs, 10.1016/0041-008x(86)90212-7
  22. HERMANS CEDRIC, BERNARD ALFRED, Lung Epithelium–specific Proteins : Characteristics and Potential Applications as Markers, 10.1164/ajrccm.159.2.9806064
  23. Ichinose Takamichi, Sagai Masaru, Biochemical effects of combined gases of nitrogen dioxide and ozone III. Synergistic effects on lipid peroxidation and antioxidative protective systems in the lungs of rats and guinea pigs, 10.1016/0300-483x(89)90196-0
  24. TAKAMICHI I, KENJI A, NOBUHIRO S, MASARU S, Biochemical effects of combined gases of nitrogen dioxide and ozone II. Species differences in lipid peroxides and antioxidative protective enzymes in the lungs, 10.1016/0378-4274(88)90074-4
  25. Krueger S., Kordecki R., Pelzl J., Bein B. K., Stray‐light correction in photoacoustic measurements of solid samples, 10.1063/1.339160
  26. Kakinoki, Yoshihiro Ohashi, Ayaki T Yasushi, Nitrogen Dioxide Compromises Defence Functions of the Airway Epithelium, 10.1080/00016489850182972
  27. Kantorski J., Tchórzewski H., The effect of serine and thiol protease inhibitors on the chemiluminescence of human neutrophils in investigationsIn Vitro, 10.1002/bio.1170070106
  28. Kawakami Masahiko, Yasui Shuji, Yamawaki Isao, Katayama Michio, Nagai Atsushi, Takizawa Takao, Structural Changes in Airways of Rats Exposed to Nitrogen Dioxide Intermittently for Seven Days: Comparison between Major Bronchi and Terminal Bronchioles, 10.1164/ajrccm/140.6.1754
  29. Lesur O, Bernard A, Arsalane K, Lauwerys R, Bégin R, Cantin A, Lane D, Clara cell protein (CC-16) induces a phospholipase A2-mediated inhibition of fibroblast migration in vitro., 10.1164/ajrccm.152.1.7541278
  30. McElroy M. C., Am. J. Physiol., 273, L1228 (1997)
  31. Moore Kevin, Roberts L. Jackson, Measurement of Lipid Peroxidation, 10.3109/10715769809065821
  32. Morrison Robert J., Singhal Sharad S., Bidani Akhil, Heming Thomas A., Awasthi Sanjay, GlutathioneS-Transferases of Rabbit Lung Macrophages, 10.1006/taap.1997.8339
  33. Neve J., Analytical chemistry in medicine and biology, 349 (1987)
  34. Paglia D. E., J. Lab. Clin. Med., 70, 158 (1967)
  35. Papi Alberto, Amadesi Silvia, Chitano Pasquale, Boschetto Piera, Ciaccia Adalberto, Geppetti Pierangelo, Fabbri Leonardo M, Mapp Cristina E, Bronchopulmonary inflammation and airway smooth muscle hyperresponsiveness induced by nitrogen dioxide in guinea pigs, 10.1016/s0014-2999(99)00302-7
  36. Pauluhn Jürgen, Acute inhalation toxicity of polymeric diphenyl-methane 4,4′-diisocyanate in rats: time course of changes in bronchoalveolar lavage, 10.1007/s002040000114
  37. Sagai Masaru, Ichinose Takamichi, Biochemical effects of combined gases of nitrogen dioxide and ozone. IV. Changes of lipid peroxidation and antioxidative protective systems in rat lungs upon life span exposure, 10.1016/0300-483x(91)90213-k
  38. Sagai Masaru, Arakawa Kenji, Ichinose Takamichi, Shimojo Nobuhiro, Biochemical effects on combined gases of nitrogen dioxide and ozone. I. Species differences of lipid peroxides and phospholipids in lungs, 10.1016/0300-483x(87)90207-1
  39. Sall J., JMP start statistics: A guide to statistical and data analysis using JMP and JMP IN software (2001)
  40. Sevanian Alex, Hacker Allen D., Elsayed Nabil, Influence of vitamin E and nitrogen dioxide on lipid peroxidation in rat lung and liver microsomes, 10.1007/bf02534941
  41. Spallholz J. E., Selenium and prevention of cancer, 1 (2001)
  42. Sun Y, Ha P-C, Butler J.A, Ou B-R, Yeh J-Y, Whanger P, Effect of Dietary Selenium on Selenoprotein W and Glutathione Peroxidase in 28 Tissues of the Rat, 10.1016/s0955-2863(97)00162-9
  43. Sunderman F. W., Clin. Chem., 16, 903 (1970)
  44. Tom W. M., Prasongwatana V., Boyde T. R. C., The effects of vitamin A nutritional status on glutathione levels and microsomal lipid peroxidation in rat lung, 10.1007/bf01952135
  45. Wasowicz W., Clin. Chem., 39, 2522 (1993)
  46. Air quality guidelines for Europe European series (2000)
  47. Yao X. L., Levine S. J., Cowan M. J., Logun C., Shelhamer J. H., Tumor Necrosis Factor- α Stimulates Human Clara Cell Secretory Protein Production by Human Airway Epithelial Cells, 10.1165/ajrcmb.19.4.3129
Bibliographic reference De Burbure, Claire ; Heilier, Jean-François ; Nève, J. ; Becker, A. ; Albrecht, C. ; et. al. Lung permeability, antioxidant status, and NO2 inhalation: A selenium supplementation study in rats.Conference on Strategies for Clean Air and Health (Rome(Italy), Nov 05-07, 2003). In: Journal of Toxicology and Environmental Health. Part A, Vol. 70, no. 3-4, p. 284-294 (2007)
Permanent URL http://hdl.handle.net/2078.1/59690