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Cryopreservation of bovine oocytes: current status and recent developments.

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Massip, Alban [UCL]

The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 degrees C. Therefore, cryopreservation of oocytes by vitrification--especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications--seems the most appropriate method.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't, Review
Publication date 2003
Journal information "Reproduction, nutrition, development" - Vol. 43, no. 4, p. 325-30 (2003)
Peer reviewed yes
issn 0926-5287
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
MESH Subject Animals ; Cattle ; Cell Survival ; Cryopreservation - instrumentation - methods - veterinary ; Cryoprotective Agents - pharmacology ; Female ; Freezing ; Oocytes - cytology
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Bibliographic reference Massip, Alban. Cryopreservation of bovine oocytes: current status and recent developments.. In: Reproduction, nutrition, development, Vol. 43, no. 4, p. 325-30 (2003)
Permanent URL http://hdl.handle.net/2078.1/9749