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Monitoring the Active Conformation of Green Fluorescent Protein (GFP) and β-Glucosidase Adsorbed on Soil Particles

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Morales Belpaire, Isabel [UCL] Gerin, Patrick A. [UCL]

In order to determine the effect of various soil components on the activity of proteins, we monitored the fluorescence and the enzymatic activity of, respectively, green fluorescent protein (GFP) and β-glucosidase adsorbed on fine soil particles. We also monitored the activity of these proteins in the presence of components that are representative of soil colloids: a montmorillonite clay, goethite and organic matter extracted from soil. Upon adsorption on clay and goethite, GFP lost its fluorescence properties while β-glucosidase suffered only a partial loss of its catalytic activity. Extractable organic matter had an inactivating role on GFP while it did not cause inactivation of β-glucosidase. When GFP and β-glucosidase adsorbed on particles from natural soil samples, their behaviour was consistent with the behaviour observed for these proteins in the presence of the separate components, suggesting that the macroscopic activity of proteins adsorbed on soil particles corresponds to an average of the activities of proteins adsorbed on a mixture of surfaces. The monitoring of the proteins on soil particles with different organic matter contents has also shown that organic matter can have different effects (protecting or inactivating) on different proteins.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "The Protein Journal" - Vol. 31, p. 84-92 (2012)
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York)
issn 1572-3887
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Proteins – Enzymes – Enzymatic activity – Fluorescence – Denaturation – Adsorption
Links
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Bibliographic reference Morales Belpaire, Isabel ; Gerin, Patrick A.. Monitoring the Active Conformation of Green Fluorescent Protein (GFP) and β-Glucosidase Adsorbed on Soil Particles. In: The Protein Journal, Vol. 31, p. 84-92 (2012)
Permanent URL http://hdl.handle.net/2078.1/97534