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Primary and secondary cartilages of the neonatal rat: the femoral head and the mandibular condyle.

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Delatte, Myriam [UCL] Von den Hoff, Johannes W van Rheden, René E M Kuijpers-Jagtman, Anne M

Primary and secondary cartilages differ in embryonic origin and in histological organization, and are generally considered to have a different mode of growth. However, few studies have directly compared the two types of cartilage of the same animal at the same age. Therefore, we analysed several histological and biochemical differences between secondary cartilage of the mandibular condyle and primary cartilage of the femoral head of 4-d-old rats. We evaluated the tissue organization, the level of DNA and glycosaminoglycan (GAG) synthesis, and the GAG and collagen content. The expression of collagen types I, II and III and of receptors for insulin-like growth factor (IGF)-I, fibroblast growth factor (FGF), and transforming growth factor (TGF)-beta were investigated by immunohistochemistry. The ex vivo DNA and GAG synthesis as well as the GAG content of femoral heads were much higher than that of mandibular condyles. Mandibular condyles expressed both collagen types I and II, while femoral heads expressed only type II collagen. In the mandibular condyles, receptors for IGF-I, FGF, and TGF-beta were observed mainly in the superficial layers, whereas they were found throughout the entire femoral head. In conclusion, major differences were found between both types of cartilage, which might be related to their specific functional demands.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2004
Journal information "European Journal of Oral Sciences (Print)" - Vol. 112, no. 2, p. 156-162 (2004)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing, Inc. (Malden)
issn 0909-8836
Publication status Publié
Affiliations UCL - MD/MDEN - Ecole de médecine dentaire et de stomatologie
UCL - (SLuc) Unité d'orthodontie et d'orthopédie dento-faciale
MESH Subject Animals ; Animals, Newborn ; Cartilage - anatomy & histology - cytology ; Cell Differentiation ; Chondrocytes - cytology ; Collagen Type I - analysis ; Collagen Type II - analysis ; Collagen Type III - analysis ; DNA - analysis ; Femur Head - anatomy & histology - cytology ; Glycosaminoglycans - analysis ; Growth Plate - anatomy & histology - cytology ; Hypertrophy ; Mandibular Condyle - anatomy & histology - cytology ; Rats ; Rats, Wistar ; Receptor, IGF Type 1 - analysis ; Receptors, Fibroblast Growth Factor - analysis ; Receptors, Transforming Growth Factor beta - analysis ; Stem Cells - cytology
Keywords Cartilage ; Mandibular condyle ; Femoral head ; Rat ; Collagen
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Bibliographic reference Delatte, Myriam ; Von den Hoff, Johannes W ; van Rheden, René E M ; Kuijpers-Jagtman, Anne M. Primary and secondary cartilages of the neonatal rat: the femoral head and the mandibular condyle.. In: European Journal of Oral Sciences (Print), Vol. 112, no. 2, p. 156-162 (2004)
Permanent URL http://hdl.handle.net/2078.1/9786