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NRAGE, a p75NTR adaptor protein, is required for developmental apoptosis in vivo.

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Bertrand, Mariève [UCL] Kenchappa, R S Andrieu, D Leclercq-Smekens, M Nguyen, H N T De Backer, O

NRAGE (also known as Maged1, Dlxin) is a member of the MAGE gene family that may play a role in the neuronal apoptosis that is regulated by the p75 neurotrophin receptor (p75NTR). To test this hypothesis in vivo, we generated NRAGE knockout mice and found that NRAGE deletion caused a defect in developmental apoptosis of sympathetic neurons of the superior cervical ganglia, similar to that observed in p75NTR knockout mice. Primary sympathetic neurons derived from NRAGE knockout mice were resistant to apoptosis induced by brain-derived neurotrophic factor (BDNF), a pro-apoptotic p75NTR ligand, and NRAGE-deficient sympathetic neurons show attenuated BDNF-dependent JNK activation. Hair follicle catagen is an apoptosis-like process that is dependent on p75NTR signaling; we show that NRAGE and p75NTR show regulated co-expression in the hair follicle and that identical defects in hair follicle catagen are present in NRAGE and p75NTR knockout mice. Interestingly, NRAGE knockout mice have severe defects in motoneuron apoptosis that are not observed in p75NTR knockout animals, raising the possibility that NRAGE may facilitate apoptosis induced by receptors other than p75NTR. Together, these studies demonstrate that NRAGE plays an important role in apoptotic-signaling in vivo.Cell Death and Differentiation advance online publication, 5 September 2008; doi:10.1038/cdd.2008.127.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 2008
Journal information "Cell death and differentiation" - Vol. 15, no. 12, p. 1921-9 (2008)
Peer reviewed yes
issn 1350-9047
Publication status Publié
Affiliation UCL
MESH Subject Trigeminal Ganglion - abnormalities ; Sympathetic Nervous System - cytology ; Receptor, Nerve Growth Factor - metabolism ; Neoplasm Proteins - deficiency - metabolism ; Mutation - genetics ; Motor Neurons - cytology ; Mice, Knockout ; Mice ; Hair Follicle - growth & development - pathology ; Gene Targeting ; Fertility ; Apoptosis ; Animals ; Adaptor Proteins, Signal Transducing - metabolism
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Bibliographic reference Bertrand, Mariève ; Kenchappa, R S ; Andrieu, D ; Leclercq-Smekens, M ; Nguyen, H N T ; et. al. NRAGE, a p75NTR adaptor protein, is required for developmental apoptosis in vivo.. In: Cell death and differentiation, Vol. 15, no. 12, p. 1921-9 (2008)
Permanent URL http://hdl.handle.net/2078.2/14199