Mesenchymal cells: metalloproteinases and adhesion on microcarriers

Mosbeux, C. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Pedregal, A. R. dos Santos [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Sousa, D. Ribeiro [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Hendric, Vincianne [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Joseph, N. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Bensellam, Mohammed [UCL] Blankaert, D. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Marique, T. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Alloin, C. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] Parent, D. [Erasme Hospital, Belgium] Liesnard, C. [Erasme Hospital, Belgium] Vooren, J. P. Van [Erasme Hospital, Belgium] Lowagie, S. [Université Libre de Bruxelles, Belgium] Werenne, J. [Laboratory of Animal Cell Biotechnology. Dept. of Bioengineering, Université Libre de Bruxelles, Belgium] et al.[show all]

The purpose of this work was to approach the development of a safe and validable process to grow the amounts of adult Mesenchymal Stem Cells (MSC) necessary for future biomedical applications. Using a single use Vue Life bag system integrated in a simple agitations bioreactor we constructed, we have studied the adhesion, migration and growth properties of MSC on different carriers (Cytodex and Bionoc) in comparison to other cells (endothelial/Kaposi sarcoma cells, CHO and HeLa) in relation to the expression of endogenous Metalloproteinases.