An anterograde rabies virus vector for high-resolution large-scale 
reconstruction of 3D neuron morphology

Haberl, MG; Viana da Silva, S; Guest, JM; Ginger, M; Ghanem, A; Mulle, C; Oberlaender, M; Conzelmann, K-K; Frick, A

doi:10.1007/s00429-014-0730-z

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An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology

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Oberlaender, M
Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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http://link.springer.com/content/pdf/10.10072Fs00429-014-0730-z.pdf
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Citation

Haberl, M., Viana da Silva, S., Guest, J., Ginger, M., Ghanem, A., Mulle, C., et al. (2015). An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology. Brain Structure and Function, 220(3), 1369-1379. doi:10.1007/s00429-014-0730-z.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002A-465E-C

Abstract

Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14 months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.