A chemogenetic approach for the optical monitoring of voltage in neurons

Sundukova, Mayya; Prifti, Efthymia; Bucci, Annalisa; Kirillova, Kseniia; Serrao, Joana; Reymond, Luc; Umebayashi, Miwa; Hovius, Ruud; Riezman, Howard; Johnsson, Kai; Heppenstall, Paul A.

doi:10.1002/anie.201812967

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

このアイテムの新しいバージョンが利用可能です:
https://pure.mpg.de/pubman/item/item_3024329_3

詳細要約

公開

学術論文

A chemogenetic approach for the optical monitoring of voltage in neurons

MPS-Authors

/persons/resource/persons203696

Johnsson, Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://onlinelibrary.wiley.com/doi/epdf/10.1002/anie.201812967
(全文テキスト（全般）)

https://doi.org/10.1002/anie.201812967
(全文テキスト（全般）)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Sundukova, M., Prifti, E., Bucci, A., Kirillova, K., Serrao, J., Reymond, L., Umebayashi, M., Hovius, R., Riezman, H., Johnsson, K., & Heppenstall, P. A. (2018). A chemogenetic approach for the optical monitoring of voltage in neurons. Angewandte Chemie International Edition, 2019(58), 1-5. doi:10.1002/anie.201812967.

引用: https://hdl.handle.net/21.11116/0000-0002-E506-5

要旨

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.