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Evidence against co-storage of enkephalins with noradrenaline in bovine adrenal medullary granules

MPG-Autoren
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Taugner,  G.
Max Planck Institute for Medical Research, Max Planck Society;

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Wunderlich,  Ilse
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Zitation

Lang, R. E., Taugner, G., Gaida, W., Ganten, D., Kraft, K., Unger, T., et al. (1982). Evidence against co-storage of enkephalins with noradrenaline in bovine adrenal medullary granules. European Journal of Pharmacology, 86(1), 117-120. doi:10.1016/0014-2999(82)90408-3.


Zitierlink: https://hdl.handle.net/21.11116/0000-0003-6C61-7
Zusammenfassung
Bovine adrenal chromaffin granules were purified and separated into adrenaline (A) and noradrenaline (NA) containing granule populations by a combination of differential and isopycnic gradient centrifugation. The distribution of [Leu5]enkephalin as measured by radioimmunoassay followed a pattern identical to adrenaline but not noradrenaline on centrifugation. This suggests that enkephalins are stored in the same type of granule where adrenaline is found or that enkephalins and adrenaline even coexist in one and the same granule. Enkephalins may therefore function as co-transmitters or co-hormones of adrenaline rather than noradrenaline in the adrenal medulla.