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Crystallization and Structure of the Photosynthetic Reaction Centres from Rhodobacter sphaeroides - wild type and mutants

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Fritzsch,  Günter
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Ermler,  Ulrich       
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Merckel,  Michael C.
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Michel,  Hartmut       
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Fritzsch, G., Ermler, U., Merckel, M. C., & Michel, H. (1996). Crystallization and Structure of the Photosynthetic Reaction Centres from Rhodobacter sphaeroides - wild type and mutants. In M.-E. Michel-Beyerle (Ed.), The Reaction Center of Photosynthetic Bacteria (pp. 3-13). Berlin, Heidelberg: Springer.


Cite as: https://hdl.handle.net/21.11116/0000-0007-5C80-1
Abstract
Trigonal crystals of the photosynthetic reaction centres from the purple bacterium Rhodobacter sphaeroides have been grown with potassium phosphate as precipitant. These crystals diffract to 2.6 Å and are suitable for a detailed structure determination. The cofactor binding is similar to that observed in the reaction centres of Rhodopseudomonas viridis. A 23 Å long water chain connects the secondary quinone QB with the cytoplasm. The structures of the mutants Thr M222 → Val, Trp M252 → Phe, and Trp M252 → Tyr show minor differences compared with the wild type. In the carotenoidless strain R26 the carotenoid-binding site is occupied by a detergent molecule.