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Structure determination of the O‐specific polysaccharides from Citrobacter O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional 1 H‐NMR spectroscopy

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Dabrowski,  Janusz
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Hauck,  Michael
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Romanowska, E., Romanowska, A., Dabrowski, J., & Hauck, M. (1987). Structure determination of the O‐specific polysaccharides from Citrobacter O4‐ and O27‐lipopolysaccharides by methylation analysis and one‐ and two‐dimensional 1 H‐NMR spectroscopy. FEBS Letters, 211(2), 175-178. doi:10.1016/0014-5793(87)81431-X.


Cite as: https://hdl.handle.net/21.11116/0000-0007-9949-B
Abstract
Using sugar and methylation analyses, and one‐ and two‐dimensional 1H‐NMR spectroscopy at 500 MHz it was established that poly‐β‐1,2‐4‐deoxy‐D‐arabinohexopyranose occurs as O‐specific chains of lipopolysaccharides in Citrobacter serotypes O4, O27 and O36. Strong serological cross‐reactivity between these serotypes is in full agreement with the chemical identity of their O‐specific polysaccharides.