A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.

Nagai, Nobuhiro; Yunoki, Shunji; Satoh, Yasuharu; Tajima, Kenji; Munekata, Masanobu

doi:10.1016/S1389-1723(05)00319-1


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A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.

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Title:	A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.
Authors:	Nagai, Nobuhiro Browse this author
	Yunoki, Shunji Browse this author
	Satoh, Yasuharu Browse this author →KAKEN DB
	Tajima, Kenji Browse this author →KAKEN DB
	Munekata, Masanobu Browse this author
Keywords:	cell sheet
	salmon atelocollagen
	fibrillar gel
	collagenase digestion
Issue Date:	2004
Publisher:	Society for Bioscience and Bioengineering, Japan
Journal Title:	Journal of Bioscience and Bioengineering
Volume:	98
Issue:	6
Start Page:	493
End Page:	496
Publisher DOI:	10.1016/S1389-1723(05)00319-1
PMID:	16233743
Abstract:	We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL cells.
Relation:	http://www.sbj.or.jp/
Relation:	http://www.sciencedirect.com/science/journal/13891723
Type:	article (author version)
URI:	http://hdl.handle.net/2115/15816
Appears in Collections:	創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永井展裕

OAI-PMH ( junii2 , jpcoar_1.0 )

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