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Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
| Title: | Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer. |
| Authors: | Sudo, Yuki Browse this author | | Iwamoto, Masayuki Browse this author | | Shimono, Kazumi Browse this author | | Kamo, Naoki Browse this author |
| Keywords: | Sensory rhodopsin | | Protein–protein interaction | | Photocycle | | Flash-photolysis | | M-intermediate | | Halobacterium salinarum |
| Issue Date: | Jul-2002 |
| Publisher: | Elsevier Science B.V. |
| Journal Title: | Journal of Photochemistry and Photobiology B Biology |
| Volume: | 67 |
| Issue: | 3 |
| Start Page: | 171 |
| End Page: | 176 |
| Publisher DOI: | 10.1016/S1011-1344(02)00322-6 |
| PMID: | 12167316 |
| Abstract: | Pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II) is a receptor of the negative phototaxis of Natronobacterium pharaonis and forms a complex with its transducer pHtrII in membranes. Flash-photolyis of a D75N mutant did not yield the M-intermediate, but an O-like intermediate is observed in a ms time range. We examined the interaction between the D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of 0.1% n-dodecyl-β-maltoside, and the association accelerated the decay of the O of D75N from 15 to 56 s−1. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and KD, the dissociation constant, were estimated. The value of n was unity and KD was estimated to 146 nM. This KD value can be considered to be the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74 (2001) 489) reported a KD of 15 μM for the interaction between the wild-type and t-Htr by means of the change in M-decay rates. Therefore, this value should be the KD value for the interaction between M of the wild-type and t-Htr. |
| Relation: | http://www.sciencedirect.com/science/journal/10111344 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/16004 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加茂 直樹
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