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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
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Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis
| Title: | Eicosapentaenoic acid suppresses ocular inflammation in endotoxin-induced uveitis |
| Authors: | Suzuki, Misa Browse this author | | Noda, Kousuke Browse this author | | Kubota, Shunsuke Browse this author | | Hirasawa, Manabu Browse this author | | Ozawa, Yoko Browse this author | | Tsubota, Kazuo Browse this author | | Mizuki, Nobuhisa Browse this author | | Ishida, Susumu Browse this author |
| Issue Date: | 17-Jul-2010 |
| Publisher: | Molecular Vision |
| Journal Title: | Molecular Vision |
| Volume: | 16 |
| Start Page: | 1382 |
| End Page: | 1388 |
| Abstract: | Purpose: To investigate the effect of eicosapentaenoic acid (EPA) on acute ocular inflammation in an animal model of endotoxin-induced uveitis (EIU). Methods: C57Bl/6 mice (6-week-old males) were orally treated with EPA at a dose of 50 mg/kg/day for 5 days. EIU was then induced in the animals by intraperitoneal injection of 160 μg lipopolysaccharide (LPS). Twenty-four hours after LPS injection, leukocyte adhesion to the retinal vasculature was evaluated by the concanavalin A lectin perfusion-labeling technique, and leukocyte infiltration into the vitreous cavity was quantified. Furthermore, the protein levels of monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, intercellular adhesion molecule-1 and phospholyrated nuclear factor (NF)-κB p65 in the retina and retinal pigment epithelium (RPE)-choroid complex were examined by enzyme-linked immunosorbent assay (ELISA). Results: At 24 h after LPS injection, the EIU animals treated with oral EPA administration showed a significant decrease in leukocyte adhesion to the retinal vessels by 43.4% (p< 0.01) and leukocyte infiltration into the vitreous cavity by 49.2% (p<0.05). In addition, EPA significantly reduced the protein levels of MCP-1 and IL-6 in the retina and the RPE-choroid complex. Furthermore, phosphorylation of NF-κB was suppressed by EPA treatment. Conclusions: Our data suggest that EPA inhibits multiple inflammatory molecules in vivo. EPA may become a novel strategy in the prevention and/or treatment of ocular inflammatory diseases. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/43846 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石田 晋
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