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Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis
| Title: | Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis |
| Authors: | Kajimoto, Kazuaki Browse this author | | Yamamoto, Masahiko Browse this author | | Watanabe, Misuzu Browse this author | | Kigasawa, Kaoru Browse this author | | Kanamura, Kiyoshi Browse this author | | Harashima, Hideyoshi Browse this author | | Kogure, Kentaro Browse this author |
| Keywords: | Liposomes | | Iontophoresis | | Transdermal delivery | | Insulin | | Diabetes |
| Issue Date: | 17-Jan-2011 |
| Publisher: | Elsevier B.V. |
| Journal Title: | International Journal of Pharmaceutics |
| Volume: | 403 |
| Issue: | 1-2 |
| Start Page: | 57 |
| End Page: | 65 |
| Publisher DOI: | 10.1016/j.ijpharm.2010.10.021 |
| PMID: | 20970487 |
| Abstract: | Iontophoresis is a promising technique for enhancing transdermal administration of charged drugs. However, conventional iontophoresis is not sufficient for effective delivery of large, hydrophilic, or electrically neutral molecules. In this study, we utilized charged liposomes as carriers, focused on a transfollicular route for delivery of the liposomes, and optimized iontophoretic conditions and lipid composition for this method in both in vitro and in vivo conditions. As a result, we identified the optimum condition (lipid composition: DOTAP/EPC/Chol = 2:2:1, current supply: 0.45 mA/cm2, duration: 1 hr) for effective iontophoretic delivery of aqueous solution, which can not be transferred into the skin without charged liposomes. We also examined the pharmacological effects of iontophoresis of liposomes encapsulating insulin (INS-lipo) using a rat model of type I diabetes. Interestingly, iontophoresis of INS-lipo onto a diabetes rat skin resulted in a gradual decrease in blood glucose levels, with levels reaching 20% of initial values at 18 hr after administration. These lower blood glucose levels were maintained for up to 24 hr. Significant amount of insulin were also detected in plasma 18 hr after iontophoresis of INS-lipo. We succeeded in developing a non-invasive and persistent transfollicular drug delivery system that used a combination of liposomes and iontophoresis. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44921 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 梶本 和昭
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